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目的:观察新降糖颗粒对2型糖尿病(type 2 diabetes mellitus,T2DM)大鼠血清空腹胰岛素水平(fasting insulin,FINS),超氧化物歧化酶(superoxide dismutase,SOD),丙二醛(malondialdehyde,MDA),C-反应蛋白(C-react protein,CRP)及肝脏葡萄糖激酶(glucokinase,GK)基因的影响,探讨其降糖机制。方法:将SD大鼠随机分为正常对照组和糖尿病造模组。采用高脂饮食联合小剂量ip链脲佐菌素(STZ)28 mg·kg-1建立2型糖尿病模型,造模成功后大鼠随机分为模型组,新降糖颗粒高、中、低剂量组(22.8,11.4,5.7 g·kg-1),二甲双胍组(0.15 g·kg-1)。正常对照组和模型组给予生理盐水,各给药组分别ig给予相应剂量药物。连续ig 5周后,氧化酶法检测大鼠空腹血糖(fasting blood-glucose,FBG)的变化;ELISA法检测大鼠血清中的FINS,CRP的变化;试剂盒检测大鼠血清中SOD,MDA的水平;RT-qPCR法检测肝脏中GK mRNA的表达。结果:与正常对照组比较,模型组中大鼠的FBG,胰岛素抵抗指数(insulin resistance index,IRI)及血清中FINS,CRP含量均显著升高(P<0.01),血清中SOD活性明显降低(P<0.01),新降糖颗粒高、中、低剂量组FBG,IRI,FINS,CRP活性均明显降低(P<0.05,P<0.01),SOD活性明显升高(P<0.05,P<0.01);模型组大鼠肝脏中GK mRNA的表达明显降低(P<0.05),新降糖颗粒中、低剂量组GK mRNA表达量显著升高(P<0.01)。结论:新降糖颗粒能明显降低T2DM大鼠血糖,其可能的作用机制是通过降低糖尿病IRI水平,增强抗氧化应激能力,减轻炎症反应,以及提高肝脏GK mRNA水平而起作用。
Objective: To observe the effect of Xinjiangtang Granule on serum fasting insulin (FINS), superoxide dismutase (SOD), malondialdehyde (MDA) in type 2 diabetes mellitus (T2DM) (MDA), C-react protein (CRP) and hepatic glucokinase (GK) gene, and to explore their hypoglycemic mechanisms. Methods: SD rats were randomly divided into normal control group and diabetic model group. High-fat diet combined with low dose of ip streptozotocin (STZ) 28 mg · kg-1 to establish type 2 diabetes model, the rats were randomly divided into model group, new hypoglycemic particles high, medium and low dose Group (22.8,11.4,5.7 g · kg-1) and metformin group (0.15 g · kg-1). The normal control group and the model group were given normal saline, and each administration group was given the corresponding dose of ig. After 5 weeks, the changes of fasting blood glucose (FBG) in rats were detected by oxidase method; the changes of FINS and CRP in serum were detected by ELISA; the contents of SOD and MDA Level; RT-qPCR method to detect the expression of GK mRNA in the liver. Results: Compared with the normal control group, FBG, insulin resistance index (IRI) and FINS and CRP levels in the model group were significantly increased (P <0.01), while the serum SOD activity was significantly decreased (P <0.01, P <0.01). The activities of FBG, IRI, FINS and CRP of XTLG in high, medium and low dose groups were significantly decreased (P <0.05, P <0.01) ). The expression of GK mRNA in the model group was significantly lower than that in the model group (P <0.05). The expression of GK mRNA in the model group was significantly increased (P <0.01). CONCLUSION: XTLP can significantly reduce the blood glucose level in T2DM rats. The possible mechanism is that XINJIANGGU granules can reduce the level of IRI, increase the anti-oxidative stress, reduce the inflammatory reaction and improve the hepatic GK mRNA level.