目的：研究一氧化氮（ＮＯ） 及一氧化碳（ＣＯ） 对碱性成纤维细胞生长因子（ｂａｓｉｃ ｆｉｂｒｏｂｌａｓｔ ｇｒｏｗｔｈ ｆａｃｔｏｒ，ｂＦＧＦ） 促平滑肌细胞（ｖａｓｃｕｌａｒｓｍｏｏｔｈ ｍｕｓｃｌｅ ｃｅｌｌｓ， ＶＳＭＣ）增殖作用的影响及其机制。方法：以３ＨＴｄＲ参入法测定平滑肌细胞增殖程度，放射活性法测定ＶＳＭＣ内蛋白磷酸化程度、蛋白激酶Ｃ（ｐｒｏｔｅｉｎ ｋｉｎａｓｅ Ｃ，ＰＫＣ） 及丝裂素活化蛋白激酶（ｍｉｔｏｇｅｎａｃｔｉｖａｔｅｄ ｐｒｏｔｅｉｎ ｋｉｎａｓｅ， ＭＡＰＫ）活性。结果：孵育２４ ｈ 后，ｂＦＧＦ刺激的ＶＳＭＣ增殖较对照组增加１．１ 倍（Ｐ＜ ０．０１），细胞内蛋白磷酸化程度增加３８．２％ （Ｐ＜ ０．０１），ＰＫＣ 及ＭＡＰＫ 活性分别增加１．５ 和２ ．５倍（ Ｐ＜ ０．０１）；ＣＯ 前体ｈｅｍｅＬｌｙｓｉｎａｔｅ（ＨＬＬｙｓ） 及ＮＯ 前体ＬＡｒｇｉｎｉｎｅ（ ＬＡｒｇ） 对静止期ＶＳＭＣ 生长无显著影响，亦未引起细胞内蛋白磷酸化、ＰＫＣ及ＭＡＰＫ 活性发生显著改变；５、１０ 、２０ μｍｏｌ·Ｌ－１ ＨＬＬｙｓ 使ｂＦＧＦ刺激的ＶＳＭＣ增殖分别减少４３．４ ％ 、４６．６％ 和４７ ．６％ Objective: To investigate the effects of nitric oxide (NO) and carbon monoxide (CO) on the proliferation of basic fibroblast growth factor (bFGF) -stimulated smooth muscle cells (VSMCs) and its mechanism. Methods: 3H-TdR incorporation was used to determine the proliferation of smooth muscle cells. The levels of phosphorylated protein kinase C (PKC) and mitogen-activated protein kinase (MAPK) )active. Results: The bFGF-stimulated proliferation of VSMC increased by 1.1-fold (P <0.01) and the level of intracellular protein phosphorylation increased by 38.2% (P <0.01) 24 h after incubation. PKC and MAPK Activity increased by 1.5 and 2, respectively. (P <0.01). CO precursor hemeLlysinate (HLLys) and NOLArginine (LArginine) had no significant effect on the growth of quiescent VSMCs, Protein phosphorylation PKC and MAPK activity changes significantly; 5,10,20 μmol·L-1 H L-Lys bFGF-stimulated VSMC proliferation decreased by 43.4%, 46.6% and 47 respectively. 6%