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目的 用表达端粒酶反义 RNA的方法研究端粒酶在喉癌细胞生长中的作用 .方法 将端粒酶反义 RNA真核表达载体 p BBS2 12 - h TR转染入人喉癌 Hep- 2细胞系中 ,采用端粒重复扩增法测定端粒酶活性 ,用 MTT法及流式细胞仪检测细胞周期 ,并用电镜观察细胞生长状况 .结果 端粒酶反义 RNA表达后能抑制 Hep- 2细胞的端粒酶活性 ,抑制细胞增殖并促进其凋亡 .结论 以端粒酶反义 RNA降低端粒酶表达的方法对人喉癌细胞系 Hep- 2的生长有明显抑制作用 .
Objective To study the role of telomerase in the growth of laryngeal carcinoma cells by expressing telomerase antisense RNA.Methods The antisense RNA of telomerase antisense RNA was transfected into human laryngeal carcinoma Hep- 2 cell line, telomerase activity was determined by telomere repeat amplification assay, cell cycle was detected by MTT assay and flow cytometry, and cell growth was observed by electron microscopy.Results The expression of telomerase antisense RNA inhibited Hep- 2 cells.Conclusion The method of inhibiting the expression of telomerase by telomerase antisense RNA can obviously inhibit the growth of human laryngeal carcinoma cell line Hep-2.