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试验采用人早幼粒白血病HL-60细胞,主要通过细胞活性丧失、染色质凝聚、核小体间DNA断裂等凋亡特征,研究了腐植酸诱导细胞凋亡的效应,观察了不同时间不同剂量(50～400μg/mL)腐植酸对细胞凋亡的影响。腐植酸诱导HL-60细胞凋亡主要与线粒体内细胞色素c的释放有关,并证明伴随着Caspase-3(天冬氨酸特异性半胱氨酸蛋白酶)的激活和PARP[poly(ADP-ribose)polymerase]的特异性蛋白断裂(一种参与凋亡细胞死亡机制的主要成分)。腐植酸诱导细胞凋亡与Bax蛋白水平相关,同时可观察到Bcl-2蛋白水平有微量降低。这些数据分析表明,腐植酸通过诱导细胞凋亡,抑制了HL-60细胞的增殖及生长,这些作用可能具有抗癌性质,对新药产品的开发有潜在帮助。
The human promyelocytic leukemia cell line HL-60 was used to study the effect of humic acid on apoptosis induced by different activities such as loss of cell activity, chromatin condensation and DNA breaks between nucleosomes. (50 ~ 400μg / mL) humic acid on apoptosis. Humic acid-induced apoptosis in HL-60 cells was mainly associated with the release of cytochrome c in mitochondria and was shown to be associated with the activation of Caspase-3 (caspase-cysteine) and PARP [poly ) polymerase], a major component involved in apoptotic cell death mechanisms. Humic acid-induced apoptosis was correlated with Bax protein level, while a slight decrease in Bcl-2 protein level was observed. These data suggest that humic acid may inhibit the proliferation and growth of HL-60 cells by inducing apoptosis. These effects may have anticancer properties and potentially help to develop new drug products.