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目的观察结膜黏膜相关淋巴组织(MALT)淋巴瘤中microRNA-150(miR-150)的表达,探讨miR-150在结膜MALT淋巴瘤中影响肿瘤细胞增殖、迁移与侵袭的机制。方法使用qPCR法检测第二军医大学长征医院收治的3例结膜MALT淋巴瘤患者肿瘤组织及瘤旁组织中miR-150及其可能的下游靶分子Cbl-b的表达。将miR-150抑制物和阴性对照转染人多发性骨髓瘤细胞株RPMI 8226,采用CCK-8法和流式细胞术研究miR-150对RPMI 8226细胞增殖和凋亡的影响,通过Transwell实验研究miR-150对RPMI 8226细胞迁移和侵袭的影响,用蛋白质印迹法检测miR-150对RPMI 8226细胞中Cbl-b表达的调控。结果与瘤旁组织相比,结膜MALT淋巴瘤组织中miR-150表达上调(P<0.05,P<0.01);抑制miR-150后,RPMI 8226细胞的增殖受到抑制,细胞凋亡明显增加,迁移和侵袭能力降低,与阴性对照组相比差异有统计学意义(P<0.05,P<0.01)。在结膜MALT淋巴瘤组织中,miR-150下游靶基因Cbl-b表达下调(P<0.01);抑制miR-150后,RPMI 8226细胞内Cbl-b蛋白的表达上调(P<0.01)。结论 MiR-150对淋巴瘤细胞的增殖、迁移和侵袭有促进作用,其表达上调参与了MALT淋巴瘤的发生。其机制可能与miR-150对下游分子Cbl-b的抑制性调控有关。
Objective To observe the expression of microRNA-150 (miR-150) in conjunctival mucosa-associated lymphoid tissue (MALT) lymphoma and to explore the mechanism of miR-150 affecting the proliferation, migration and invasion of tumor cells in conjunctival MALT lymphoma. Methods The qPCR method was used to detect the expression of miR-150 and its possible downstream target molecule Cbl-b in tumor tissues and tumor-adjacent tissues of 3 patients with conjunctival MALT lymphoma admitted to Changzheng Hospital of Second Military Medical University. The effects of miR-150 on the proliferation and apoptosis of RPMI 8226 cells were studied by CCK-8 assay and flow cytometry. The effects of miR-150 inhibitor and negative control on the proliferation and apoptosis of RPMI 8226 cells were investigated by Transwell assay The effect of miR-150 on the migration and invasion of RPMI 8226 cells was examined by western blotting. The effect of miR-150 on the expression of Cbl-b in RPMI 8226 cells was detected by Western blot. Results Compared with the adjacent tissue, the expression of miR-150 in conjunctival MALT lymphoma was up-regulated (P <0.05, P <0.01). After miR-150 was inhibited, the proliferation of RPMI 8226 cells was inhibited and the apoptosis was significantly increased And invasive ability decreased, compared with the negative control group, the difference was statistically significant (P <0.05, P <0.01). In conjunctival MALT lymphoma tissues, the expression of Cbl-b was down-regulated (P <0.01), while the expression of Cbl-b was up-regulated in RPMI 8226 cells after miR-150 was inhibited (P <0.01). Conclusions MiR-150 can promote the proliferation, migration and invasion of lymphoma cells. MiR-150 is involved in the development of MALT lymphoma. The mechanism may be miR-150 on the downstream molecules Cbl-b inhibition of regulation.