目的观察巢蛋白(nestin)阳性细胞在发育早、中期胎儿肝中的形态及分布,探讨其在肝发育中的作用。方法取10~20周胎儿肝组织,常规石蜡切片,地高辛标记nestin寡核苷酸探针,原位杂交组织化学法显示netin阳性细胞。结果nestin阳性细胞主要分布在肝界板处或发育中的胆管周围,细胞体积较小,细胞核呈圆或卵圆形,核大,常见双核。结论肝内存在一定数量的nestin阳性细胞,该细胞具有较强的分裂增殖能力,可能是胚胎早期发育过程中隐藏在机体各种组织中的一类具有相同功能的细胞,在日后的肝细胞修复及肿瘤的发生中起着重要的作用。 Objective To observe the morphology and distribution of nestin positive cells in fetal liver during the early and middle stages of development and to explore its role in liver development. Methods Fetal liver tissues were obtained from 10 to 20 weeks old, paraffin sections were stained with digoxigenin and labeled with nestin oligonucleotide probe. In situ hybridization histochemistry showed netin positive cells. Results Nestin positive cells mainly distributed around the bile duct or the developing bile duct. The cells were small in size, with a round or oval nucleus, large nucleus and common dual nucleus. Conclusion There are a number of nestin-positive cells in the liver, which have a strong ability of division and proliferation. They may be a kind of cells with the same function hidden in various tissues of the body during early embryo development. In the future, hepatocytes repair And tumor occurrence plays an important role.