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为了了解γ 线照射是否影响体外培养的树突状细胞的表型与功能 ,利用含rhGM CSF(80 0U/ml)和rhIL 4(5 0 0U/ml)的RPMI 16 4 0培养基从多发性硬化症病人的外周血单个核细胞 (PBMNC)中诱生树突状细胞 (DC)。在培养的第 6天加入 5 μg/ml的脂多糖继续培养 2 4小时促使DC完全成熟。于第 7天收获DC并等分成几部分 ,一部分未经γ 线照射的DC用作对照组 ,其他部分的DC分别用 2 5Gy和 30Gy剂量的γ射线照射。流式细胞术分析DC的表面分子并测定DC细胞刺激同源T细胞增殖的能力。结果表明 ,γ 线照射减少树突状细胞CD86 ,CD80和HLA DR表达 ,尤其是CD86分子的表达 (P =0 .0 0 72 )。人DC能有效地刺激同源T细胞的增殖 ,但是同未经照射的DC相比 ,照射的DC刺激T细胞增殖的能力显著降低。结论 :γ 线照射不仅影响DC的表型 ,而且影响它的功能
In order to understand whether γ-ray irradiation affects the phenotype and function of dendritic cells cultured in vitro, RPMI1640 medium containing rhGM CSF (80 0U / ml) and rhIL 4 (500U / ml) Dendritic cells (DCs) are induced in peripheral blood mononuclear cells (PBMNC) of sclerotic patients. On the sixth day of culture, 5μg / ml lipopolysaccharide was added to continue culturing for 24 hours to promote DC to mature completely. DCs were harvested on day 7 and aliquoted into portions. A portion of DCs without gamma irradiation was used as a control group, and the other portions of DCs were irradiated with gamma rays of 25 Gy and 30 Gy, respectively. Flow cytometry was used to analyze surface molecules of DCs and determine the ability of DC cells to stimulate proliferation of cognate T cells. The results showed that γ ray irradiation reduced the expression of CD86, CD80 and HLA DR, especially CD86 molecules in dendritic cells (P = 0.0072). Human DC effectively stimulated homologous T cell proliferation, but the ability of irradiated DCs to stimulate T cell proliferation was significantly reduced compared to non-irradiated DCs. Conclusion: γ-ray irradiation not only affects the phenotype of DC, but also affects its function