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目的 观察低氧对大鼠心脏内皮素 1(ET 1)水平的调节作用 ,以及血管钠肽 (VNP)对这一过程的影响。方法 将大鼠随机分为 4组 :对照组、低氧组、VNP(75 μg·kg-1)组和低氧 +VNP(2 5～ 75 μg·kg-1)组 ,采用放射免疫的方法测定大鼠心脏cGMP和ET 1水平 ,并以原位杂交的方法分析ET 1的mRNA水平。结果 低氧使大鼠心脏ET 1及其mRNA的水平升高 (P <0 0 5 ,vs对照组 ) ;VNP(5 0、75 μg·kg-1)使低氧大鼠心脏ET 1及其mRNA的水平降低 (P <0 0 5 ,vs低氧组 )。对照组和低氧组的心脏cGMP水平没有差异 ,而VNP组和低氧 +VNP(5 0、75 μg·kg-1)组的心脏cGMP水平高于对照组和低氧组 (P <0 0 5 )。结论 低氧可以使大鼠心脏ET 1及其mRNA的水平增加 ,而VNP可以抑制这一过程
Objective To observe the regulatory effect of hypoxia on the level of endothelin 1 (ET 1) in rat heart and the effect of vascular natriuretic peptide (VNP) on this process. Methods The rats were randomly divided into 4 groups: control group, hypoxia group, VNP (75 μg · kg -1) group and hypoxia + VNP group (25 ~ 75 μg · kg -1) The level of cGMP and ET 1 in rat heart was measured and the level of ET 1 mRNA was analyzed by in situ hybridization. Results Hypoxia increased the level of ET 1 and its mRNA in rat heart (P <0.05, vs control group); VNP (50, 75 μg · kg -1) mRNA level decreased (P <0 05, vs hypoxia group). There was no difference in cardiac cGMP levels between the control group and the hypoxia group, while the cGMP level in the heart of the VNP group and hypoxia + VNP group (50, 75 μg · kg -1) was higher than that of the control group and the hypoxia group 5). Conclusion Hypoxia can increase the level of ET 1 and its mRNA in rat heart, while VNP can inhibit this process