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目的：研究脑钠肽（ｂｒａｉｎｎｅｔｒｉｕｒｅｔｉｃｐｅｐｔｉｄｅ，ＢＮＰ）的镇痛作用及机制。方法：在小鼠热板、扭体和甲醛试验上，分别观察用药后舔足潜伏期、扭体数及疼痛评分的变化。一氧化氮（ＮＯ）用ｇｒｅｅｎ法测定。结果：ＢＮＰ（２５，５０，１００μｇ·ｋｇ－１，ｉｐ）能显著延长小鼠舔足潜伏期，并呈量效关系，其ｉｐＥＤ５０为３８．６μｇ·ｋｇ－１；ｉｐ２５、５０μｇ·ｋｇ－１ＢＮＰ显著减少小鼠扭体数和脑组织中ＮＯ含量；ｉｐ５０μｇ·ｋｇ－１和ｉｃｖ２μｇ·ｋｇ－１ＢＮＰ均可显著抑制甲醛致小鼠疼痛，降低疼痛评分，进一步发现ＢＮＰ的镇痛作用可被ＥＧＴＡ加强，ＣａＣｌ２拮抗，但维拉帕米可部分逆转ＣａＣｌ２对ＢＮＰ镇痛的拮抗。结论：ＢＮＰ有显著镇痛作用，其镇痛作用可被Ｃａ２＋影响。
Objective: To study the analgesic effect and mechanism of brain natriuretic peptide (BNP). Methods: On the hot plate, writhing and formaldehyde test in mice, the change of latency, writhing number and pain score of licking foot were observed. Nitric oxide (NO) is determined by the green method. Results: BNP (25, 50, 100μg · kg-1, ip) could significantly prolong the licking latency of mice and showed a dose-response relationship with ipED50 of 38.6μg · kg-1; ip25 and 50μg · kg-1BNP Reduce the number of writhing in mice and NO content in brain tissue; ip50μg · kg-1 and icv2μg · kg-1BNP can significantly inhibit formaldehyde-induced pain in mice and reduce pain scores, and further found that the analgesic effect of BNP can be enhanced by EGTA, CaCl2 antagonism, but verapamil partially reversed CaCl2 antagonism of BNP analgesia. Conclusion: BNP has a significant analgesic effect and its analgesic effect can be affected by Ca2 +.