在拉丁美洲地区,由于粘膜皮肤利什曼病、内脏利什曼病常与恰加斯氏病的分布重叠,虽然过去曾应用过一些不同的血清学方法,但有不同程度的交叉反应。作者以活的克氏锥虫鞭毛体经培养大量收集后,以pH7.2的0.01MPBS缓冲液洗3次,并在PBS中提取48小时后离心取得抗原。利什曼抗原以巴西利什曼和杜氏利什曼的鞭毛体经PBS洗3次后冰冻干燥,再将 In Latin America, visceral leishmaniasis often overlaps with the distribution of Chagas disease due to mucosal cutaneous leishmaniasis, and although some different serological methods have been used in the past, there are varying degrees of cross-reaction. After a large quantity of live Trypanosoma cruzi flagella body was collected by culture, the cells were washed three times with 0.01 M PBS pH7.2 and extracted in PBS for 48 hours and then centrifuged to obtain antigens. Leishmania antigen Leishmaniasis and Leishman’s flagella body washed three times with PBS and then freeze-dried, and then