论文部分内容阅读
采用扩增片段长度多态性技术(AFLP)对6个紫菜品系进行遗传差异性分析。结果表明,在42对引物中,有6对能得到重复性较好的多态性条带,共扩增出141条谱带,其中133条为多态性谱带,多态位点的比例达94.33%。基于引物扩增结果计算遗传相似性,结果显示不同紫菜品系间存在差异。UPGMA、NJ法聚类分析结果表明,条斑紫菜品系Y001和Y-L0602遗传相似系数高,聚为一类;条斑紫菜和坛紫菜遗传重组品系Y-H034和H-Y035聚为一类,之后与Y-H017品系聚集;坛紫菜H017品系与其他供试品系遗传距离较远,聚类在最外侧。各品系间遗传距离与其选育方法有关,筛选获得的遗传重组后代表现出与条斑紫菜更近的亲缘关系。
The genetic diversity of six seaweed cultivars was analyzed using amplified fragment length polymorphism (AFLP). The results showed that among the 42 pairs of primers, 6 pairs of primers were able to obtain better repeatability of the polymorphic bands, a total of 141 bands were amplified, of which 133 were polymorphic bands, the proportion of polymorphic loci Up to 94.33%. Based on the results of primer amplification, genetic similarity was calculated and the results showed that there were differences among different seaweed strains. The results of UPGMA and NJ showed that the genetic similarity coefficients of Y003 and Y-L0602 were high and clustered together. The genetic recombination lines Y-H034 and H-Y035 clustered into one category, Afterwards, it was clustered with Y-H017 strain. The genetic distance between algae H017 and other tested lines was far and clustered on the outermost side. The genetic distance between the lines is related to the breeding methods. The genetic recombination obtained after screening shows the closer genetic relationship with Porphyra yezoensis.