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为探讨苦瓜降糖有效成分的分离纯化,观察其在体外对四氧嘧啶破坏的胰腺β细胞(HIT-T15细胞)的保护修复作用,用SP阳离子交换色谱法对苦瓜抽提液(Momordicacharantiaextract,MCE)的活性组分进行了分离纯化,并用MTT法对经四氧嘧啶破坏前后的胰腺β细胞存活率进行检测,从而考察了MCE的各组分对胰岛β细胞的保护和修复功能,此外还测定了各组分的抗氧活性。结果表明:SP阳离子交换树脂的分离各组分对胰腺β细胞的存活都有促进作用,其中P1、P3、P6组分的效果显著,又以P6组分的效果最佳。相对正常对照组,经P1、P3和P6组分保护并修复的细胞残存率分别为78.64%、89.20%和94.22%;经P1、P3和P6组分保护的细胞残存率分别达59.03%、61.07%和66.16%;经P1、P3和P6组分修复的细胞残存率分别达69.52%、76.32%和77.19%。P1、P3和P6组分的SOD活性分别为22.85、29.98和27.53u/mL。结论:苦瓜抽提液中的3个组分具有一定的SOD活性,在适当的浓度范围内,均具有明显减弱四氧嘧啶对胰腺β细胞的损伤和改善受损的胰腺β细胞的功能。
In order to investigate the isolation and purification of the effective components of bitter melon hypoglycemic, the protective and repairing effect of alloxan-induced destruction of pancreatic β-cells (HIT-T15 cells) was observed in vitro. Momordica charantia extract (MCE) was obtained by SP cation exchange chromatography. The active components were isolated and purified, and the viability of pancreatic beta cells before and after destruction with alloxan was examined by MTT assay to examine the protective and repair functions of the various components of MCE on pancreatic islet β cells. The antioxidant activity of the components. The results showed that the separation of SP cation exchange resin components can promote the survival of pancreatic β cells, among which P1, P3 and P6 have significant effects, and P6 components have the best effect. Compared with the normal control group, the residual rates of cells preserved and repaired by P1, P3, and P6 components were 78.64%, 89.20%, and 94.22%, respectively; the residual rates of cells protected by P1, P3, and P6 components were 59.03%, 61.07, respectively. % and 66.16%; the residual rate of cells repaired by P1, P3 and P6 components was 69.52%, 76.32% and 77.19%, respectively. The SOD activities of P1, P3, and P6 components were 22.85, 29.98, and 27.53 u/mL, respectively. Conclusion: The three components in bitter melon extract have a certain SOD activity. Within a suitable concentration range, all of them have a significant effect on alleviating pancreatic β-cell injury and improving the function of damaged pancreatic β-cells.