为了明确氟对人牙乳头间充质细胞和分泌碱性磷酸酶活性的影响 ,体外培养人牙乳头间充质细胞 ,给予不同浓度氟处理 :0 μmol,10 μmol(0 .2× 10 - 6 g/ L) ,5 2 μmol (1.0× 10 - 6 g/ L) ,2 6 3μmol(5 .0× 10 - 6 g/ L) ,1315 μmol (2 5×10 - 6 g/ L) ,2 6 30 μmol(5 0× 10 - 6 g/ L) ,用四唑盐比色法 (MTT法 )测定细胞活力 ,酶联免疫法检测碱性磷酸酶活性 ,结果显示 ,当氟浓度为 2 6 3μmol时 ,培养 2 4 h后 ,细胞活性明显抑制 ,AL P活性无明显改变 ;当氟浓度为 1315 μmol和2 6 30 μmol时 ,培养 12 h细胞活性明显抑制 ,分泌的 AL P活性升高 ,培养 72 h时 2 6 30 μmol氟浓度组几乎无活细胞 ,而AL P活性仍较对照组高 ,由此提示 ,氟作用于人牙乳头细胞时 ,细胞活性与 AL P活性升高不一致 In order to clarify the effect of fluoride on human dental papilla mesenchymal cells and secretion of alkaline phosphatase, cultured human dental papilla mesenchymal cells were treated with different concentrations of fluoride (0 μmol, 10 μmol (0.2 × 10 -6 g / L), 5 2 μmol (1.0 × 10-6 g / L), 263μmol (5.0 × 10-6 g / L), 1315 μmol 6 30 μmol (50 × 10-6 g / L). The activity of alkaline phosphatase was assayed by MTT method. The results showed that when the fluoride concentration was 26 At the concentration of 1315 μmol and 26 30 μmol, the activity of ALP was significantly inhibited and the secreted ALP activity increased at the concentration of 3 μmol for 24 h. However, Almost no viable cells were found in the 26 6 μmol concentration fluoride-treated group at 72 h, while ALP activity was still higher than that of the control group, suggesting that the cell activity is not consistent with the increased AL P activity when fluoride is applied to human dental papilla cells