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目的对北极海参硫酸软骨素(Holothuria mexicana)和低分子量糖的体内抗血栓活性和机制进行研究比较。方法以海参硫酸软骨素(CS)和低分子量海参硫酸软骨素(LCS)为研究对象,使用电刺激颈动脉血栓模型,分析比较不同分子量海参硫酸软骨素对血栓形成时间、血栓烷(TXB2)、6-酮-前列腺素(6-ketoPGF1α)、组织途径抑制因子(TFPI)、血友病因子(VWF)含量和凝血途径的影响。结果海参硫酸软骨素和低分子量硫酸软骨素均有较强的抗血栓活性。在有效抗栓浓度下,海参硫酸软骨素和低分子量硫酸软骨素体内抗栓作用机理为:海参硫酸软骨素能够作用于内源凝血途径,并且通过抑制TXA2的生成和调控VWF含量下降来抑制血栓生成,但是其对外源凝血途径TFPI和PGI2无明显作用。同时,海参硫酸软骨素和低分子量硫酸软骨素的抗栓活性相比较得出,当硫酸软骨素分子量下降时,其抗血栓能力也会下降,但是其体内抗血栓作用机制不变。结论北极海参硫酸软骨素及其低分子量糖具有较强的体内抗血栓活性。
Objective To study the antithrombotic activity and mechanism of Arctic sea cucumber Holothuria mexicana and low molecular weight sugar in vivo. Methods The rat model of carotid artery thrombosis was established by using the sea cucumber chondroitin sulphate (CS) and low molecular weight sea cucumber chondroitin sulphate (LCS) as the research object. The effects of different molecular weight chondroitin sulphate on the thrombosis time, TXB2, 6-keto-PGF1α, TFPI, VWF and coagulation pathways. Results sea cucumber chondroitin sulfate and low molecular weight chondroitin sulfate have strong antithrombotic activity. Under effective antithrombotic concentration, the antithrombotic mechanism of sea cucumber chondroitin sulfate and low molecular weight chondroitin sulfate in vivo is that chuanxiong sulfate chuancin can act on endogenous coagulation pathway, and inhibit thrombus by inhibiting TXA2 production and regulating VWF content decrease But it had no significant effect on the extrinsic coagulation pathway TFPI and PGI2. In the meantime, the antithrombotic activity of sea cucumber chondroitin sulfate and low molecular weight chondroitin sulfate was compared. When the molecular weight of chondroitin sulfate decreased, its anti-thrombotic ability also decreased, but its anti-thrombotic mechanism remained unchanged in vivo. Conclusion Arctic sea cucumber chondroitin sulfate and its low molecular weight sugar have strong antithrombotic activity in vivo.