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目的:研究紫花牡荆素(Casticin)对肝癌HepG2细胞增殖抑制和凋亡诱导的作用,并探讨其作用机制。方法:用终浓度为0、0.5、1.0、2.0umol/L的Casticin作用于HepG2细胞,于12、24、48h后采用MTT法检测细胞增殖抑制率;Hoechst33342核染色,观察细胞形态学变化;24h后收集各组肝癌HepG2细胞,流式细胞术检测细胞周期及凋亡率;RT-PCR检测survivin mRNA表达。结果:MTT法检测显示,Casticin对肝癌HepG2细胞有增殖抑制作用,并存在浓度和时间依赖关系;Hoechst33342染色后,可见核染色质凝集,凋亡细胞呈致密浓染,与对照组相比,Casticin处理后凋亡细胞比例增加;Casticin作用24h后,细胞被阻滞于G2/M期,随药物质量浓度的增加,细胞凋亡率逐渐增加;RT-PCR结果显示,Casticin下调肝癌HepG2细胞survivin mRNA表达。结论:Casticin在体外对肝癌HepG2细胞有明显的增殖抑制和凋亡诱导作用,初步推断Casticin诱发肝癌细胞凋亡与其对survivin基因表达的抑制有关。
Objective: To investigate the effect of Casticin on the proliferation inhibition and apoptosis induction of hepatocellular carcinoma HepG2 cells, and to explore its mechanism of action. METHODS: HepG2 cells were treated with Casticin with final concentrations of 0, 0.5, 1.0, and 2.0 umol/L. After 24 h, 24 h and 48 h, MTT assay was used to detect the inhibition of cell proliferation. Hoechst 33342 nuclear staining was used to observe the morphological changes of cells. Hepatocellular carcinoma HepG2 cells were harvested. The cell cycle and apoptosis rate were detected by flow cytometry. The expression of survivin mRNA was detected by RT-PCR. RESULTS: MTT assay showed that Casticin inhibited the proliferation of hepatocellular carcinoma HepG2 cells in a concentration- and time-dependent manner. After Hoechst33342 staining, nuclear chromatin condensation was observed and the apoptotic cells were densely stained. Compared with the control group, Casticin The proportion of apoptotic cells increased after treatment. After 24 hours of Casticin, the cells were arrested in G2/M phase. With the increase of drug concentration, the apoptosis rate increased gradually. RT-PCR showed that Casticin down-regulated the expression of survivin mRNA in HepG2 cells. expression. Conclusion: Casticin can inhibit proliferation and induce apoptosis of hepatocellular carcinoma HepG2 cells in vitro. It is concluded that the apoptosis of hepatoma cells induced by Casticin is related to the inhibition of survivin gene expression.