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构建过表达白细胞介素-12(interleukin-12,IL-12)的THP-1单核系肿瘤细胞模型,从形态学、巨噬细胞表面标志表达水平及吞噬功能3方面探究过表达IL-12能否调节单核系肿瘤细胞向巨噬细胞分化。结果发现,与空白和空载组相比,IL-12组的THP-1细胞界限不明显,散在细胞增多;呈圆形或不规则圆形,偶见毛刺状突起;核仁模糊或消失,核染色质条索状浓集;CD68 mRNA和蛋白表达量均升高(P<0.05)。与空白组相比,CD11b mRNA和蛋白表达量升高不明显(P>0.05),但72 h CD11b蛋白表达量明显高于相同培养条件下的空载组(P<0.05)。培养72 h后各组细胞吞噬能力比较,IL-12组吞噬率(36.7±1.2)%高于空白组(15.7±3.1)%和空载组(28.0±1.5)%,差异有统计学意义(P<0.05)。上述结果提示,过表达IL-12可以促进单核系肿瘤细胞向巨噬细胞分化。
To establish a THP-1 monocytogenetic tumor cell model that overexpresses interleukin-12 (IL-12), and to explore the effects of overexpression of IL-12 on morphology, expression level of macrophage surface markers and phagocytic function Can regulate monocyte line of tumor cells to macrophage differentiation. The results showed that, compared with the blank and no-load group, the IL-12 group THP-1 cell line is not obvious, scattered cells; round or irregular round, occasionally burr-like protrusions; nucleolus fuzzy or disappear, Nuclear chromatin cord-like concentration; CD68 mRNA and protein expression were increased (P <0.05). Compared with the blank group, the expression of CD11b mRNA and protein was not significantly increased (P> 0.05), but the expression of CD11b protein at 72 h was significantly higher than that of the control group (P <0.05). The phagocytic rate of IL-12 group (36.7 ± 1.2)% was significantly higher than that of blank group (15.7 ± 3.1)% and no-load group (28.0 ± 1.5)% after 72 h culture, the difference was statistically significant P <0.05). The above results suggest that overexpression of IL-12 can promote monocyte-derived tumor cells to differentiate into macrophages.