在浸渍鱼类受精卵的媒液中加入抗冻结剂(Ch_3)_2so_4,再加入进一步提高其防冻解冻作用的补助剂—葡萄糖或仔牛血清,能使低温保存后的精卵孵化率几乎高达100％。在以葡萄糖作为补助剂添加的情况下,就是在(CH)_2So_4波度为2.1m(摩尔)的无机盐溶液中再加入葡萄糖,在葡萄糖浓度为5.6mM时,孵化率有40—57％,在波度 Adding antifreeze agent (Ch_3) _2so_4 into the liquid of the zygote of fish fishers, adding the supplementary agent of glucose or baby bovine to further increase the antifreeze thawing effect, the hatching rate of the sperm egg after cryopreservation is almost up to 100% . In the case of adding glucose as a supplement, glucose is added to an inorganic salt solution having a (CH) _2So_4 wave number of 2.1 m (mol) and a hatching rate of 40 to 57% at a glucose concentration of 5.6 mM, In the wave