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以2年生四季竹为试材,利用开顶式气室(OTCs)熏蒸法模拟大气CO2和O3浓度升高情景,研究了高浓度CO2(700μmol·mol-1)和高浓度O3(100nmol·mol-1)及其复合作用对四季竹叶片膜脂过氧化、抗氧化酶及渗透调节物质的影响。结果表明:高浓度O3处理30d,四季竹叶片抗氧化酶活性和渗透调节物质含量提高,清除活性氧能力增强,并未出现明显的膜脂过氧化,四季竹能耐受短期的O3胁迫。高浓度O3处理90d,四季竹叶片抗氧化酶活性显著降低,膜脂过氧化程度加剧,膜结构破坏,发生严重的氧化损伤;高浓度CO2处理30d,四季竹叶片抗氧化酶活性和渗透调节物质含量提高,能维持活性氧平衡,存在一定的生理适应期。而高浓度CO2处理90d,四季竹叶片抗氧化酶活性和活性氧含量降低,可溶性糖和可溶性蛋白含量升高,有利于四季竹生长;高浓度O3和CO2复合作用下,相对于单因素高浓度O3处理,四季竹叶片能够维持较高的抗氧化酶活性和渗透调节物质含量,有效地调节活性氧产生与清除的平衡,膜脂过氧化不明显,说明高浓度CO2一定程度上可缓解高浓度O3对四季竹所造成的生理伤害。
Taking the biennial bamboo as test material, the open air chamber (OTCs) fumigation method was used to simulate the atmospheric CO2 and O3 concentrations. The effects of high CO2 concentration (700μmol · mol-1) and high concentration O3 (100nmol · mol -1) and their combined effects on membrane lipid peroxidation, antioxidant enzymes and osmotic adjustment substances of Allium mongolicum. The results showed that after treated with high concentration of O3 for 30 days, the activities of antioxidant enzymes and osmoregulation substances in the leaves of All Seasons rose, and the capability of scavenging reactive oxygen species increased. There was no significant membrane lipid peroxidation. At high concentration of O3 for 90 days, the activities of antioxidant enzymes in the leaves of All Seasons were significantly decreased, the degree of membrane lipid peroxidation increased, the membrane structure was destroyed and serious oxidative damage occurred. High concentration CO2 treatment for 30 days showed that the activities of antioxidant enzymes and osmotic adjustment substances Content increased, to maintain the balance of reactive oxygen species, there is a certain physiological adaptation period. However, under the high CO2 concentration for 90 days, the activities of antioxidant enzymes and reactive oxygen species decreased and the content of soluble sugar and soluble protein increased, which was beneficial to the growth of Four Seasons Bamboo. Under the action of high concentration of O3 and CO2, O3 treatment, bamboo leaves can maintain a high level of antioxidant enzyme activity and osmotic adjustment substance content, effectively regulate the balance between generation and elimination of reactive oxygen species, lipid peroxidation is not obvious, indicating that high concentrations of CO2 to some extent, can alleviate the high concentration O3 on the four seasons caused by the physiological damage bamboo.