目的调查艾滋病病毒Ⅰ型(HIV-1)感染相关的CCR5、CCR2及SDF1等位基因,在广西壮族和汉族普通人群中的多态性及其流行病学意义。方法在广西天等县和南宁市,选取150名壮族和90名汉族健康人作为研究对象,采集外周血,提取基因组DNA,用聚合酶链反应(PCR)扩增CCR5、CCR2及SDF1基因的特定片段。直接根据PCR扩增结果分析CCR5基因多态性,运用限制性片段长度多态性(RFLP)技术分析CCR2及SDF1的多态性。结果全部研究对象的CCR5基因均为野生型,未发现CCR5Δ32突变;CCR2-64I等位基因频率在壮、汉族普通人群中分别为25.7%、26.1%;SDF1-3′A等位基因频率分别为27.7%和27.2%。结论广西壮、汉族普通人群缺乏艾滋病抗性的CCR5Δ32等位基因,而CCR2-64I和SDF1-3’A等位基因频率较高。该研究为深入研究广西壮、汉族普通人群对于HIV-1感染的遗传易感性,以及对艾滋病疫情和病程的影响提供了比较全面、可靠的数据。 Objective To investigate the polymorphisms of CCR5, CCR2 and SDF1 alleles associated with HIV-1 infection in the general population of Zhuang and Han in Guangxi and its epidemiological significance. Methods Totally 150 Zhuang and 90 Han nationality healthy subjects were selected as experimental subjects in Guangxi Tianxi County and Nanning City. Peripheral blood was collected and genomic DNA was extracted. The specific CCR5, CCR2 and SDF1 genes were amplified by polymerase chain reaction (PCR) Fragment. The CCR5 gene polymorphism was directly analyzed according to the result of PCR amplification and the polymorphism of CCR2 and SDF1 was analyzed by restriction fragment length polymorphism (RFLP). Results All the CCR5 genes were wild type and no CCR5Δ32 mutation was found. The frequency of CCR2-64I allele was 25.7% and 26.1% in the general population of Zhuang and Han respectively. The frequency of SDF1-3’A allele was 27.7% and 27.2%. Conclusion The general population of Zhuang and Han in Guangxi lacks AIDS-resistant CCR5Δ32 allele, while the frequency of CCR2-64I and SDF1-3’A allele is higher. The study provides more comprehensive and reliable data for further study on the genetic susceptibility to HIV-1 infection in the general population of Zhuang and Han nationality in Guangxi as well as the impact on AIDS epidemic and disease course.