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在油菜素甾醇(brassinosteroids,BRs)化合物中,油菜素内酯(brassinolide,BL)具有活性最高、广谱和无毒等显著特点,而且具有改良植物株型、提高抗逆性等功效。根系是植物吸收水分和矿质元素的主要器官,因此阐明油菜素内酯调控根系发育的遗传、生理和生化机制,有利于更有效地利用BRs激素,实现株型的定向设计。该研究利用叶面喷施的方法分析油菜素内酯对根系侧根、根毛发育的影响;利用植物显微技术分析油菜素内酯对根系侧根结构及发育的作用;利用高压液相色谱法检测油菜素内酯对根系内其他植物激素含量的影响;利用蛋白质组学技术鉴定受油菜素内酯调控的蛋白质,分析油菜素内酯调控根系发育的生化机制。研究表明,一定浓度的油菜素内酯促进种子根、侧根、根毛的发生;提高根系细胞分裂素和赤霉素含量;可能通过调控逆境相关蛋白质来提高植物的抗逆性。
Among brassinosteroids (BRs) compounds, brassinolide (BL) is the most active, broad-spectrum and nontoxic product. It has the effects of improving the plant type and improving the resistance to stress. The root system is the main organ that plants absorb moisture and mineral elements. Therefore, elucidating the genetic, physiological and biochemical mechanism of brassinosteroid regulation of root development is beneficial to the more efficient use of BRs hormones to achieve directional design of plant type. In this study, the effects of brassinolide on root and root hairs development of root system were analyzed by foliar spraying. The effect of brassinolide on root structure and development of root system was analyzed by plant microscopy. The effect of brassinolide on hormone content of other plant roots was studied. The protein regulated by brassinolide was identified by proteomics and the biochemical mechanism of brassinosteroid regulation of root development was analyzed. The results showed that a certain concentration of brassinosteroid promoted the occurrence of seed root, lateral root and root hairs, increased the contents of cytokinin and gibberellin in roots, and increased plant stress resistance through regulating stress-related proteins.