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目的 了解产青霉素酶淋病奈瑟菌（ＰＰＮＧ） 株的耐药性特征、耐药与质粒携带类型之间的相互关系以及追踪耐药菌株的来源。方法 对本地区１９９６ 年５ ～７ 月分离的４８ 株ＰＰＮＧ 标本进行了分析。用琼脂扩散法和琼脂稀释法测定标本对１１ 种抗生素的敏感性。碱变性加溶菌酶法对标本进行质粒抽提。电洗脱法回收５－３ｋｂ 和７－４ｋｂ 质粒，用Ｂａｍ ＨⅠ单切酶和Ｂａｍ ＨⅠ＋ Ｐｓｔ Ⅰ、Ｂａｍ ＨⅠ＋ ＢｇＬⅠ双切酶酶切分析。结果 ４８ 株ＰＰＮＧ 株对青霉素Ｇ、四环素和氟哌酸耐药率较高，分别为１００ ％ 、９３－７ ％ 和８９－５８ ％ ，对其余抗生素较敏感。４８ 株ＰＰＮＧ 菌株中４７ 株被检出耐药性质粒，但耐药性质粒酶切片段多少不一，具有明显的多态性。结论 耐药性质粒酶切分析为追踪耐药性质粒来源及耐药菌株的流行趋势提供了流行病学信息。
Objective To understand the drug resistance characteristics of penicillinase-producing Neisseria gonorrhoeae (PPNG) strains, the relationship between drug resistance and plasmid-borne types, and to trace the origin of resistant strains. Methods 48 PPNG specimens from May to July in 1996 were analyzed. The sensitivity of the specimens to 11 antibiotics was determined by agar diffusion and agar dilution. Alkalization and lysozyme enzymatic extraction of the specimens. The 5-3 kb and 7-4 kb plasmids were recovered by electroelution, and digested with BamHI mono-enzyme and BamHI + PstI, BamHI + BgLI. Results The 48 strains of PPNG showed high resistance rates to penicillin G, tetracycline and norfloxacin, which were 100%, 93-7% and 89-58% respectively, which were sensitive to the remaining antibiotics. Forty-seven isolates of 48 isolates of PPNG were detected as drug-resistant plasmids, but the fragments of drug-resistant plasmids were different in number and had obvious polymorphism. Conclusions Resistant plasmid DNA analysis provided epidemiological information for tracing the source of drug-resistant plasmids and the prevalence of drug-resistant strains.