采用细胞共培养方式和免疫化学染色方法 ,研究骨髓基质细胞对神经干细胞分化为神经元、星形胶质细胞和寡突胶质细胞的影响。实验发现 ,体外培养的中脑神经干细胞在与成年大鼠骨髓基质细胞共培养 7d后 ,在神经干细胞后代中神经元比例可达 38.6 %± 10 .8% ,明显高于自然分化组 2 0 % ,提示骨髓基质细胞提供的微环境可明显提高神经干细胞后代中神经元的比例。 The effects of bone marrow stromal cells on the differentiation of neural stem cells into neurons, astrocytes and oligodendrocytes were studied by cell co-culture and immunochemical staining. The results showed that the percentage of neurons in the progeny of neural stem cells reached 38.6% ± 10.8% after being cultured with adult rat bone marrow stromal cells for 7 days, which was significantly higher than that of the natural differentiation group (20% , Suggesting that the microenvironment provided by bone marrow stromal cells can significantly increase the proportion of neurons in progeny of neural stem cells.