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为从微生态角度探索接种生防放线菌剂对魔芋根域微生物区系的影响,以生防放线菌娄彻氏链霉菌(Streptomyces rochei)、濑里予链霉菌(S.senoensis)和M(肉质链霉菌S.carnosus和密旋链霉菌S.pctum按质量比1:1的固态发酵混合制剂)为接种剂,采用基质拌菌法接种生防菌剂进行盆栽试验,通过稀释平板法测定魔芋根区、根表土壤和根内放线菌、细菌及真菌数量并采用分子生物学技术对优势真菌和细菌进行分类鉴定.结果显示:(1)3种菌剂接种164 d,魔芋根域检出的接入放线菌活菌数量高达106 CFU g-1以上,根表土壤中放线菌数量较对照增加24.6%-263.1%;(2)供试3种菌剂接种164 d后,魔芋根域真菌数量减少18.9%-100.0%,且接种处理魔芋根域有害优势真菌腐皮镰孢菌(Fusarium solani)和红球丛赤壳菌(Nectria haematococca)较未接种对照大幅度降低;(3)不同菌剂接种处理,魔芋根区土壤和根内细菌数量较对照减少21.5%-73.3%,但根内芽孢杆菌(Bacillus sp.)数量增加414.0%-1015.0%.本研究表明供试生防菌具有较稳定的定殖能力,亦能改善魔芋根域土壤微生物区系.
In order to explore the effect of biocontrol actinomycete inoculation on the microflora in the root zone of konjac rootlets from the perspective of microecology, Streptomyces rochei, S.senoensis and M (Streptomyces succulentus S. carnosus and S. compactii S.pctum by mass ratio of 1: 1 solid state fermentation mixed preparation) as inoculant, the use of substrate mixed bacteria biocidal agent inoculated pot experiment, by dilution plate method The number of actinomycetes, bacteria and fungi in root zone, rhizosphere and root of konjak were determined and the dominant fungi and bacteria were identified by molecular biology techniques.The results showed that: (1) The three inoculants were inoculated 164 days, The number of viable actinomyces in the field was higher than 106 CFU g-1, and the number of actinomycetes in root surface soil increased by 24.6% -263.1% compared with the control. (2) , The number of fungi in the root zone of konjac decreased by 18.9% -100.0%, and the fungi Fusarium solani and Nectria haematococca in the root zone of konjac root inoculation treatment significantly decreased compared with the non-inoculated control; (3) Inoculation with different fungicides, the number of bacteria and bacteria in the root and root of konjac root zone decreased compared with the control 21.5% -73.3%, but the number of Bacillus sp. Increased by 414.0% -1015.0% .This study shows that the test for the prevention and treatment of bacteria with a more stable colonization ability, but also can improve the root zone of konjac soil microbial flora .