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目的 探讨L arg对人肾系膜细胞增殖的影响及其作用机制。方法 分别运用MTT法及细胞免疫组化、流式细胞仪等技术研究L arg对人肾系膜细胞增殖的影响。 结果 L arg抑制人系膜细胞增殖呈浓度、时间依赖性 ;L arg组系膜细胞PCNA表达增加 ,但细胞绝对数下降 ;流式细胞仪检测显示 ,L arg干预后大量的细胞阻滞在S期 ,L arg的浓度越高 ,阻滞作用越明显。结论 L arg对人系膜细胞增殖具有抑制作用 ,其机制可能与抑制细胞有丝分裂有关。
Objective To investigate the effect of L arg on proliferation of human mesangial cells and its mechanism. Methods The effects of L arg on the proliferation of human renal mesangial cells were studied by MTT, cell immunohistochemistry and flow cytometry, respectively. Results L arg inhibited the proliferation of human mesangial cells in a time-and concentration-dependent manner. The expression of PCNA increased in mesangial cells in L arg group but the absolute number of cells decreased. Flow cytometry showed that a large number of cell blocks were blocked by L arg in S The higher the concentration of L arg, the more obvious the blocking effect. Conclusion L arg has an inhibitory effect on the proliferation of human mesangial cells, which may be related to the inhibition of cell mitosis.