A Comparative Study of SSR Diversity in Chinese Major Rice Varieties Planted in 1950s and in the Rec

来源 :Rice Science | 被引量 : 0次 | 上传用户:fang19902009
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
Forty pairs of SSR markers were used to compare the genetic diversity changes in 151 Chinese major rice varieties planted in 1950s and in the recent ten years. Of 40 SSR loci, 39 were found to be polymorphic while one locus (RM479) monomorphic. A total of 213 alleles were identified from the 39 polymorphic loci. The average number of alleles per locus (Na) was of 5.5, ranging from 2 to 11. Nei’s gene diversity index (He) varied drastically among loci from 0.309 at RM174 to 0.869 at RM418, with an average value of 0.649. There existed significant difference in SSR allelic diversity between indica and japonica subspecies, and indica had more variation than japonica both in Na and He. By comparison with the genetic changes in Na and He, it was revealed that the varieties planted in 1950s had more alleles and higher He than those in the recent ten years both for indica and japonica rices. The difference between two subspecies for Na was significant in a tendency over time (indica: z = 2.677, P = 0.007; japonica: z = 3.441, P = 0.001), but not significant for He (indica: z = 1.471, P = 0.141; japonica: z = 1.932, P = 0.053). Analysis of molecular variance (AMOVA) indicated that there existed significant difference (P < 0.05) in genetic variation between the two periods, of which more genetic variation was contributed by indica (Fst = 0.050) and japonica (Fst = 0.082) subsets. Using locus-by-locus AMOVA procedure, significant genetic differentiations were observed in 13 loci (RM21, RM128, RM147, RM169, RM190, RM221, RM231, RM251, RM253, RM317, RM341, RM418, and RM478) for indica varieties and 11 loci (RM101, RM135, RM152, RM159, RM169, RM190, RM251, RM253, RM311, RM418, and RM478) for japonica ones between the two periods. It was found some alleles had been lost in current major rice varieties as comparing with those in 1950s. Therefore, it should be necessary to exploit more alien elite genetic resources for extension of genetic background in current rice breeding program. Forty pairs of SSR markers were used to compare the genetic diversity changes in 151 Chinese major rice varieties planted in 1950s and in the recent ten years. Of 40 SSR loci, 39 were found to be polymorphic while one locus (RM479) monomorphic. A total of 213 alleles were identified from the 39 polymorphic loci. The average number of alleles per locus (Na) was of 5.5, ranging from 2 to 11. Nei’s gene diversity index (He) varied drastically among loci from 0.309 at RM174 to 0.869 at RM418 , with an average value of 0.649. There existed significant difference in SSR allelic diversity between indica and japonica subspecies, and indica had more variation than japonica both in Na and He. By comparison with the genetic changes in Na and He, it was revealed that the varieties planted in 1950s had more alleles and higher He than those in the recent ten years both for indica and japonica rices. The difference between two subspecies for Na was significant in a tendency over time (indica: z = 2. (Indica: z = 1.471, P = 0.141; japonica: z = 1.932, P = 0.053). Analysis of molecular variance (AMOVA ) indicated that there existed significant difference (P <0.05) in genetic variation between the two periods, of which more genetic variation was contributed by indica (Fst = 0.050) and japonica (Fst = 0.082) subsets. Using locus- by- locus AMOVA (RM21, RM128, RM147, RM169, RM190, RM221, RM231, RM251, RM253, RM317, RM341, RM418, and RM478) for indica varieties and 11 loci (RM101, RM135, RM152 It was found some alleles had been lost in current major rice varieties as comparing with those in 1950s. It, therefore, it should found some alleles had been lost in current major rice varieties as comparing with those in 1950s. be necessary to exploit more alien elite genetic resources for extension of genetic background in current rice breeding prog raNo.
其他文献
随着经济的发展,我国需要的能源也是逐渐增多,而在我国能源的主要来源于煤炭的燃烧,因此为了使我国的能源得于充分利用,为了使煤炭更科学的提供能源,在这里我们主要研究了煤矿的开
我是密云古北口镇旅游招商办主任,27年前入党。我从写入党申请书到入党,经历了7年时间。这期间,让我收获最大的,是认识到工作好不等于就能入党,入党不是等价交换。入党是一次
在诸多的网站中,媒体网站是新闻信息传播的主体,媒体网站在经营过程中始终关注自己网站在全球、全国或同类网站中的排名情况、页面到达率、平均页面浏览数等指标。媒体网站的
“姐姐,帮我插上电视的电源,好吗?”一个可爱的小妹妹对我说。“好啊!”我用刚洗过的湿手拿起绝缘皮已经有点破的插头。“应该找人换换这插头了!这里的设备也太差了!”我这样
土壤中N的转化过程包括N的矿化、固定、硝化过程。各个过程相互联系,共同构成一个紧密的整体。N的矿化与固定过程可以通过影响硝化过程底物以及产物的浓度影响硝化作用的强度
思绪如一叶小舟,飘来飘去又飘到你的信笺上,再次读起来,一遍又一遍。那种如丝如缕的心思,令我不得不用心思考你这个朋友,对着你远去的方向,我想知道,你在故乡还好吗?一直不理
水稻是三大粮食作物之一,2016年我国水稻种植面积0.3亿公顷,占全国粮食作物种植面积的26.7%,占全国农作物总种植面积的18.2%,我国一半以上的人口以稻米作为主食,因此,水稻生产对保障国家安全具有重要意义。氮肥是影响作物生长的三要素之一,科学合理施用氮肥不仅对保障水稻产量、而且对提高稻米品质并减少氮素向环境排放等均具有十分重要的意义。当前,我国水稻氮肥用量过多、施用方式不合理,并引发出一系列
艺术传播即指借助于一定的物质媒介和传播方式,将艺术信息或作品传递给接受者的过程。艺术接受即指在传播的基础上,以艺术作品为对象、以鉴赏者为主体,积极能动的消费、鉴赏
4月19日,由市委宣传部、市直机关工委、市新闻出版局联合举办的主题为“悦读好书,共享精品”——北京市直机关“读书、荐书、评书”活动正式启动。这项活动是今年4月份“悦读
陕西师范大学美术学院研究生郑付忠认为:由崔尔平校点的2006年版《历代书法论文选》收录了米芾《海岳名言》,其中有这样的一句话颇具争议:“友仁等古人书,不知此学吾书多,小