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目的观察基因重组hTFF3对严重烧伤所致肠黏膜损害的治疗作用。方法采用30%体表面积Ⅲ度烧伤小鼠模型,随机分成对照组、烧伤组和烧伤后hTFF3治疗(TFF3)组,TFF3组伤后1 h开始hTFF3灌胃,剂量为1 mg/kg,烧伤组和对照组灌胃等量生理盐水,各组每天灌胃1次,连续5 d。观察烧伤后肠组织病理学改变,肠黏膜肠绒毛高度、隐窝深度及病死率。结果烧伤小鼠每天给予1 mg/kg的hTFF3灌胃,显著降低肠黏膜受损程度,肠黏膜病理改变明显减轻,烧伤组以出血、坏死和溃疡为主,TFF3组以充血、水肿为主,同时肠黏膜厚度、绒毛高度、隐窝深度明显增加,小鼠5 d病死率有所下降(27.3%vs 45.6%)。结论重组hTFF3对烧伤后肠道损伤具有明显的治疗作用,能促进受损黏膜屏障的重建。
Objective To observe the therapeutic effect of gene recombinant hTFF3 on intestinal mucosal damage caused by severe burns. Methods Thirty (30%) body surface area of third degree burn mice were randomly divided into control group, burn group and burn treated group (TFF3). TFF3 group was given hTFF3 intragastrically at 1 hour after injury and the dose was 1 mg / kg. The rats in the control group were given the same amount of normal saline by intragastric administration once a day for 5 consecutive days. The histopathological changes of intestinal tissue were observed after burn, the intestinal villus height, crypt depth and mortality were observed. Results Burn mice were given intragastric administration of 1 mg / kg hTFF3 orally, significantly reducing the damage of intestinal mucosa, and significantly reducing the pathological changes of intestinal mucosa. Bleeding, necrosis and ulcer were the main cause in burn group, At the same time, intestinal mucosal thickness, villus height and crypt depth were significantly increased. The 5-day mortality of mice decreased (27.3% vs 45.6%). Conclusion Recombinant hTFF3 has a significant therapeutic effect on post-burn intestinal injury and can promote the reconstruction of damaged mucosal barrier.