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本实验室从胡黄连根茎分离到1,6-di-O-caffeoyl-β-D-glucopyranoside单体Caffeoyl Glycoside(CG),体外试验测定其对小鼠免疫细胞功能的影响。通过MTT法检测CG对Balb/c小鼠脾淋巴细胞增殖,NK细胞对K562细胞杀伤活性的影响,以及小鼠腹腔巨噬细胞(PMΦ)能量代谢水平;CG对PMΦ吞噬中性红能力的影响。CG在一定剂量范围内单独或者协同非特异性丝裂原(Con A或LPS)作用均能够显著增强小鼠脾淋巴细胞、PMΦ的增殖能力(P<0.05或P<0.01),CG单独作用就能显著提高NK对K562细胞的杀伤活性(P<0.05或P<0.01)。CG在体外对小鼠主要免疫细胞的增值和功能方面具有显著的促进作用(P<0.05或P<0.01)。研究结果显示CG具有特异性与非特异性的免疫增强作用。
In this laboratory, the isolated 1,6-di-O-caffeoyl-β-D-glucopyranoside monomer Caffeoyl Glycoside (CG) was isolated from rhizomes of Hu Huang Lian and its effect on mouse immune cell function was determined in vitro. The effect of CG on the proliferation of spleen lymphocytes of Balb/c mice, the killing activity of NK cells on K562 cells, and the energy metabolism of mouse peritoneal macrophages (PMΦ) were detected by MTT assay; the effect of CG on the phagocytic neutral red ability of PMΦ . The effects of CG alone or synergistic with non-specific mitogen (Con A or LPS) on the proliferation of splenic lymphocytes and PMΦ in mice within a certain dose range (P<0.05 or P<0.01), CG alone The killing activity of NK on K562 cells was significantly increased (P<0.05 or P<0.01). CG played a significant role in promoting the proliferation and function of mouse primary immune cells in vitro (P<0.05 or P<0.01). The results of the study showed that CG has specific and non-specific immunoenhancing effects.