【摘 要】
：
Cell polarity is essential for spatially regulating of physiological processes in metazoans by which hormonal stimulation-secretion coupling is precisely coupled for tissue homeostasis and organ communications.However,the molecular mechanisms underlying e
【机 构】
：
MOE Key Laboratory for Membraneless Organelles & Cellular Dynamics and Hefei National Laboratory for
论文部分内容阅读
Cell polarity is essential for spatially regulating of physiological processes in metazoans by which hormonal stimulation-secretion coupling is precisely coupled for tissue homeostasis and organ communications.However,the molecular mechanisms underlying epithelial cell polarity establishment remain elusive.Here,we show that septin cytoskeleton interacts with catenin complex to organize a functional domain to separate apical from basal membranes in polarized epithelial cells.Using polarized epithelial cell monolayer as a model system with transepithelial electrical resistance as functional readout,our studies show that septins are essential for epithelial cell polarization.Our proteomic analyses discovered a novel septin-catenin complex during epithelial cell polarization.The functional relevance of septin-catenin complex was then examined in three-dimensional (3D) culture in which suppression of septins resulted in deformation of apical lumen in cysts,a hallmark seen in polarity-deficlent 3D cultures and animals.Mechanistically,septin cytoskeleton stabllizes the association of adherens catenin complex with actin cytoskeleton,and depletion or disruption of septin cytoskeleton liberates adherans junction and polarity complexes into the cytoplasm.Together,these findings reveal a previously unrecognized role for septin cytoskeleton in the polarization of the apical-basal axis and lumen formation in polarized epithelial cells.
其他文献
前陆盆地以蕴藏构造油气藏为主,岩性地层油气藏在以往被认为难以形成规模资源量.以库车坳陷东部中生界为例,在大量露头、钻井、地震和测井资料叠合分析的基础上,分析出吐格尔明大型背斜构造为古新世和上新世继承性基底冲断作用的产物,其变形主压应力场的方位为NNE--SSW向;依奇克里克背斜构造为基底卷入的冲断构造,其褶皱南翼相对简单,为迪北斜坡带,北翼呈低角度北倾,变形主压应力场的方位为NNW--SSE向.库车坳陷东部中—下侏罗统和上三叠统发育巨厚层煤系腐殖型和暗色泥岩腐泥型烃源岩,沉积中心分布在库车河地区、迪北地区
Rapid climate change has provided the opportunity for many species to learn to adapt fast enough to modify their range distribution.The positive and negative responses of the species in terms of distribution are related to parameters such as kind of speci
Accumulation and losses of soil organic carbon (SOC),total nitrogen (TN) and sulphur (S)influence food security and global warming.Therefore,their spatial distribution and variability at regional scale,and their relation to topographical variables are of
建立了制动盘有限元模型,仿真得到制动盘前五阶弹性模态;对制动盘进行模态试验,阐述了制动盘模态试验的参数选择、测点布置、模态置信度判断准则,并通过综合频率响应函数和模态置信准则验证了试验结果的可靠性;仿真与试验得到的制动盘模态振型、模态频率等结果误差很小,二者基本一致.结果表明,所建立的制动盘有限元模型可用于约束状态下制动盘振动和噪声性能的预测.
The rheological behaviors of highly concentrated fine particle suspensions(clay-silt-water mixtures)and coarse particle suspensions(coarse particles within a fine particle suspension)were investigated in this study.Experimental results demonstrated that t
The Central Himalayan region is vulnerable to the adverse effects of climate change and characterized by regional climatic conditions.The livelihood of the mountain communities across the Himalaya is at risk owing to the consequences of variable precipita
将可再生能源发电丰沛期的电量用于电解水制氢并存储被认为是应对可再生能源发电不稳定缺憾的重要解决方案之一.氢气的比能在所有的燃料中最高,但其能量密度较低,存储一直是世界性难题,大量存储则更加困难.随着氢气存贮技术的不断进步,地下存储或地质存储被视为大规模储氢技术最具可行性的发展方向.
Landsat-8 spectral values have been used to map the earth\'s surface information for decades.However,forest types and other land-use/land-cover(LULC) in the mountain terrains exist on different altitudes and climatic conditions.Hence,spectral informatio
Glaciers in the central Qilian Mountains provide important water resources for the arid Hexi corridor and Qaidam Basin;however,changes in these glaciers interact with climate change.Twenty-four bi-static image pairs of TerraSAR-X add-on for Digital Elevat
In the past several decades,the trend of rainfall have been significantly increasing in the Qinghai-Tibet Plateau,which inevitably leads to a change in the surface energy balance processes and thermal-moisture status of the permafrost active layers.Howeve