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[目的]研究肝纤维化大鼠与肝脏局部肾素-血管紧张素系统(RAS)的关系,丹参煎液的抗肝纤维化作用及对CCl4肝纤维化大鼠RAS的影响。[方法]采用CCl4造模的同时,分别给以丹参煎液及氯沙坦灌服,共6周,光镜下观察病理改变;检测血清丙氨酸氨基转移酶(ALT)、天冬氨酸转氨酶(AST)、碱性磷酸酶(ALP)水平;血清透明质酸(HA)、层黏连蛋白(LN)和血管紧张素Ⅱ(AngⅡ)的水平;检测大鼠肝组织中-αSMA蛋白和AngⅡ1型受体(AT1R)mRNA表达。[结果]丹参煎液可促使大鼠肝组织的炎症和纤维化程度减轻;使肝组织中血浆AngⅡ水平降低(P<0.01);AT1R mRNA、-αSMA蛋白表达降低(P<0.05,<0.01),肝功能改善(P<0.05,<0.01);肝组织纤维化程度减轻(P<0.05,<0.01)。[结论]丹参煎液具有一定的保护肝细胞、改善肝功能、抗肝纤维化的作用。其抗肝纤维化作用的机制之一可能与其降低肝纤维化大鼠血浆AngⅡ水平,抑制肝组织AT1R的基因表达有关。
[Objective] To investigate the relationship between the local renin-angiotensin system (RAS) and hepatic fibrosis in rats and the anti-hepatic fibrosis of Radix Salviae Miltiorrhizae decoction and its effect on the RAS of CCl4-induced hepatic fibrosis in rats. [Method] The model of CCl4 was established, and the Salvia miltiorrhiza decoction and losartan were given respectively for 6 weeks. Pathological changes were observed under the light microscope. Serum levels of alanine aminotransferase (ALT), aspartate (AST), alkaline phosphatase (ALP), serum hyaluronic acid (HA), laminin (LN) and angiotensin Ⅱ (AngⅡ) Ang Ⅱ type 1 receptor (AT1R) mRNA expression. [Result] Salvia miltiorrhiza decoction could promote the alleviation of inflammation and fibrosis in rat liver tissue, decrease the level of Ang Ang in liver tissue (P <0.01), and decrease the expressions of AT1R mRNA and -αSMA protein (P <0.05, <0.01) , Liver function improved (P <0.05, <0.01), liver fibrosis reduced (P <0.05, <0.01). [Conclusion] Danshen decoction has some functions of protecting hepatocytes, improving liver function and preventing liver fibrosis. One of the mechanisms of its anti-hepatic fibrosis may be related to its role in reducing plasma AngⅡ level and inhibiting AT1R gene expression in hepatic fibrosis rats.