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应用酶联免疫吸附法(ELISA)和聚合酶链反应法(PCR)联合对406份晚孕妇女静脉血及324份新生儿脐带血的弓形体IgG、IgM、DNA片段进行检测,结果表明孕妇弓形体感染率为15.8%,新生儿感染率为5.9%。证明PCR法阳性率高于ELISA法测IgM(P<0.05)。提出在条件许可医院PCR法可代替ELISA法而做为治疗指标。对母婴弓形体IgM或PCR阳性者采用螺旋霉素或磺胺嘧啶、乙胺嘧啶、叶酸等联合治疗,并长期随访患者,以此减少弓形体对母婴的危害。达到优生优育的目的
Serum IgG, IgM and DNA fragments of Toxoplasma gondii were detected by ELISA and polymerase chain reaction (PCR) in 406 pregnant women with venous blood and 324 neonates. The results showed that the arched The rate of systemic infection was 15.8% and the rate of neonatal infection was 5.9%. The positive rate of PCR was higher than that of ELISA (P <0.05). It is proposed that the hospital PCR method can be used as a therapeutic index in place of the ELISA method. Toxoplasma gondii or PCR-positive spiramycin or sulfadiazine, pyrimethamine, folic acid and other combination therapy, and long-term follow-up of patients, in order to reduce the toxoplasmosis of maternal and child hazards. To achieve the purpose of prenatal and postnatal care