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应用Fourier红外光谱(FTIR)分析方法, 对354个宫颈脱落细胞的普查样本进行分析. 根据970和1 170 cm-1吸收峰出现与否, 可将谱图分为两大类: T1和T2. T1占83. 1%, 被诊断为正常细胞或在正常范围内的良性变化的细胞(PapⅠ). T2占16. 9%, 其中, 被诊断为PapⅠ和不正常的样品分别占28. 9%和71. 1%。不正常的样品中有3例(占5. 0%), 分别诊断为严重炎症、宫颈疤痕和宫颈糜烂. 结果表明: (1) FTIR谱学分析结果与细胞学方法检测结果基本一致. (2) FTIR谱学方法对监测细胞的改变更灵敏. 从正常到病变细胞, 谱图的变化呈现多样化和渐进性. 巴氏分级相同的样本的FTIR 谱图间仍存在着可识别的差异性. (3) FTIR谱图提示的疾病状态比细胞学方法检测的早. 上述结果提示FTIR对宫颈病变的早期诊断具有一定的应用价值, 可望成为宫颈病变普查的重要手段.
Fourier transform infrared (FTIR) analysis was used to analyze 354 samples of cervical exfoliated cells. Spectra were classified into two categories based on the presence or absence of absorption peaks at 970 and 1 170 cm-1: T1 and T2. T1 accounted for 83.1%, was diagnosed as normal cells or in the normal range of benign cells (PapI). T2 accounted for 16.9%, of which, were diagnosed as PapI and abnormal samples accounted for 28.9% And 71.1%. Three of the abnormal samples (5.0%) were diagnosed as severe inflammation, cervical scar and cervical erosion. The results showed that: (1) The results of FTIR spectroscopy were basically consistent with the results of cytological methods. (2 The FTIR spectroscopy method is more sensitive to monitoring cell changes. From normal to diseased cells, the changes in the spectra are diversified and progressive. There are still identifiable differences among the FTIR spectra of samples of the same Pap scale. (3) FTIR spectra suggest that the disease state is earlier than that detected by cytological methods. The above results suggest that FTIR has a certain application value for the early diagnosis of cervical lesions, and is expected to be an important means for the investigation of cervical lesions.