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目的:观察木犀草素对哮喘小鼠转录因子GATA-3表达的影响。方法:将30只BALB/c小鼠随机分为正常对照组、哮喘气道重塑组、木犀草素干预组,每组10只;鸡卵清蛋白致敏和激发建立哮喘小鼠气道重塑模型;HE染色观察各组气道炎症发生及气道结构改变;采用Western blot及Real time PCR技术检测治疗前后哮喘小鼠肺组织中GATA-3蛋白和GATA-3 mRNA的表达。结果:HE染色提示哮喘组出现黏膜下层和平滑肌增厚,气道管腔狭窄,大量炎细胞浸润的表现,木犀草素组上述改变较哮喘组为轻;Western blot结果显示哮喘组小鼠肺组织GATA-3蛋白表达较对照组为高(P<0.01),木犀草素治疗组表达量低于哮喘组(P<0.01)。Real time PCR结果与Western blot结果一致。结论:木犀草素干预可下调哮喘引起的GATA-3的表达;木犀草素抑制GATA-3表达可能是其缓解气道炎症的机制之一。
Objective: To observe the effect of luteolin on the transcription factor GATA-3 expression in asthmatic mice. METHODS: Thirty BALB/c mice were randomly divided into normal control group, asthma airway remodeling group, and luteolin-treated group, 10 in each group; chicken ovalbumin sensitized and challenged to establish airway remodeling in asthmatic mice. HE staining was used to observe the airway inflammation and airway structure changes in each group. Western blot and Real time PCR were used to detect the expression of GATA-3 protein and GATA-3 mRNA in asthmatic mice before and after treatment. Results: HE staining showed that submucosa and smooth muscle thickening, airway lumen stenosis, and large number of inflammatory cell infiltration in the asthma group. The luteolin group was lighter than the asthma group; Western blot results showed that the asthma group mice lung tissue The expression of GATA-3 protein was higher than that of the control group (P<0.01). The expression level of luteolin group was lower than that of the asthma group (P<0.01). Real time PCR results are consistent with Western blot results. Conclusion: Luteolin intervention can down-regulate the expression of GATA-3 induced by asthma. Luteolin inhibits GATA-3 expression may be one of the mechanisms of its remission of airway inflammation.