从患者皮损处皮屑及活检组织中分离出的暗纹节菱孢Arthriniumphaeospermum（Corda）Ellis菌株制成混悬菌液，用兔、豚鼠及小鼠（LACA）进行动物毒理实验。表皮直接涂抹法接种，四周后未见皮肤损害；划破表皮涂沫法及皮下注射法接种，两周后实验动物的皮肤都出现浸润性斑块、小给节、皮下脓肿及脱毛的症状，对照组动物正常。再用腹腔注射法接种，小鼠两周后死亡，病解所见，腹膜、肠系膜、腹壁及肝等处，均出现散在的灰白色小脓肿；心、肺、肝、脾及肾均有病变。豚鼠两周后仍存活，当即处死解剖所见与小鼠病解改变相同。对实验动物皮损、腹膜、肠系膜、胃及肝等处的小脓肿组织进行了病理检查，结果发现皮损及器官组织均有明显的病理浸润，由多种细胞构成的浸润性肉芽肿。在病理浸润内可见大小不等的球形、卵圆形、棱形及晶体形的棕色、红色孢子．其病理改变与患者皮损病理改变相一致，其不同点是实验动物有小脓肿，肉芽肿内可见多核巨噬细胞，而在病人皮损中很少见到。 Animal virulence experiments were performed on rabbits, guinea pigs and mice (LACA) using suspension cultures of strains of Rhizoctonia Arthriniumphaeospermum (Corda) Ellis isolated from skin lesions and biopsies of patients. Epidermis direct smear method of inoculation, no skin damage after four weeks; scratched epidermal smear method and subcutaneous injection of vaccination, two weeks after the experimental animals were infiltrative plaques on the skin, small section, subcutaneous abscess and hair removal symptoms, Control animals were normal. Then inoculated intraperitoneal injection, the mice died after two weeks, the disease solution seen, peritoneum, mesentery, abdominal wall and liver, etc., were scattered gray small abscess; heart, lung, liver, spleen and kidney lesions. Guinea pigs survived two weeks later, immediately sacrificed anatomical findings and mouse disease solution the same change. Pathological examination was performed on small abscesses in the skin, peritoneum, mesentery, stomach and liver of experimental animals. The results showed that pathological infiltration and pathological infiltration of the skin lesions and organs were found, and infiltrative granulomas were formed by many kinds of cells. Visible in the pathological infiltration of spherical, oval, prismatic and crystal brown, red spores. The pathological changes consistent with pathological changes in patients with lesions, the difference is that experimental animals have small abscesses, granulomas can be seen within the multinucleated macrophages, and rarely seen in patients with lesions.