本文对可移植性小鼠肺腺癌(LA-795)细胞增殖动力学的研究采用~3H-TdR单次脉冲标记的方法进行。腹腔注射~3H-TdR后不同时间解剖小鼠取新鲜瘤组织,制成病理切片,做放射自显影试验,置4℃条件下暴光16天,然后显影定影,H、E染色。结果进行银颗粒计数,并以数学方程进行计算求出有关细胞动力学参数:Li32.3％Mi1.7％LMi10％,Tg_1 2.83小时 Te_2 4.8小时Tm0.37小时 Tc15小时 Ts7小时 GF69.2％。 The study of the proliferation kinetics of transplantable mouse lung adenocarcinoma (LA-795) cells was performed using a single pulse labeling method of ~3H-TdR. After 3 hours of intraperitoneal injection of ~3H-TdR, the mice were dissected and fresh tumor tissues were dissected. The pathological sections were made and autoradiographs were performed. Exposures were performed at 4°C for 16 days, followed by fixation, H, and E staining. Results Silver particles were counted and calculated using mathematical equations to calculate relevant cell kinetic parameters: Li 32.3% Mi 1.7% LMi 10%, Tg 1. 2.83 hours Te 2 4.8 hours Tm 0.37 hours Tc 15 hours Ts 7 hours GF 69.2%.