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目的探讨人类白细胞抗原-G(HLA-G)与胎膜早破(PROM)关系及临床意义。方法随机选取2011年7月至2013年7月临产前行剖宫产分娩的初产妇,检测肘静脉血、羊水及胎膜,分为正常完好胎膜组、足月胎膜早破组和足月前胎膜早破组。应用双抗体夹心酶联免疫吸附实验测定组对象血清、羊水中HLA-G的浓度,比较各组间的相关性。结果正常完好胎膜产妇体内的HLA-G水平以及表达率均比胎膜早破产妇高,并且无感染的正常妊娠产妇胎膜的HLA-G表达率明显比亚临床感染要高,两者经检验均具显著性差异(P<0.05)。结论 HLA-G在胎盘中的低表达可能与孕妇感染有关,而HLA-G在胎盘中的低表达可能又与胎膜早破的发生有关,这对于HLA-G在PROM上的研究有着非常重要的意义。
Objective To investigate the relationship between human leukocyte antigen-G (HLA-G) and premature rupture of membranes (PROM) and its clinical significance. Methods The primiparous women were randomly selected from July 2011 to July 2013 before cesarean delivery. The elbow venous blood, amniotic fluid and fetal membranes were measured and divided into normal intact fetal membranes, full-term premature rupture of membranes and foot Premature rupture of membranes group. The concentration of HLA-G in the serum and amniotic fluid of the subjects was measured by double-antibody sandwich enzyme-linked immunosorbent assay (ELISA), and the correlation between the two groups was compared. Results The normal and intact maternal women with HLA-G levels and the expression rate were higher than the premature rupture of the maternal membranes, and non-infected normal pregnancy maternal fetal membrane HLA-G expression was significantly higher than the sub-clinical infection, both Tests were significant differences (P <0.05). Conclusion The low expression of HLA-G in placenta may be related to the infection of pregnant women, while the low expression of HLA-G in placenta may be related to the occurrence of premature rupture of membranes, which is very important for the research of HLA-G in PROM Meaning.