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目的 :探讨人牙周膜干细胞(human periodontal ligament stem cells,hPDLSCs)条件培养液(conditioned medium,CM)对舌癌Tca-8113细胞增殖及迁移能力的影响。方法 :从健康成人牙周膜组织中分离并培养hPDLSCs。第3代hPDLSCs融合达80%时更换为无血清培养液,继续培养24 h后收集上清液,即获得hPDLSCs-CM。舌癌Tca-8113细胞分别在常规培养液(对照组)和含50%hPDLSCs-CM的培养液(CM处理组)中培养,然后采用MTT法检测细胞增殖活性,FCM法分析细胞周期变化,Transwell迁移实验检测细胞迁移能力的变化。结果 :分离培养的hPDLSCs表达干细胞标志蛋白Stro-1和CD146,且具有明显的克隆形成及多向分化能力。将hPDLSCs-CM与舌癌Tca-8113细胞共培养至第3、5和7天时,CM处理组的细胞增殖活性均高于未处理对照组(P<0.01)。培养至第4天时,CM处理组的细胞增殖指数(proliferative index,PI)较对照组明显升高(P<0.01)。培养24 h后,CM处理组穿膜细胞数明显高于对照组(P<0.01)。结论 :hPDLSCs-CM可以促进舌癌Tca-8113细胞增殖,并增强该细胞的迁移能力。
Objective: To investigate the effect of conditioned medium (CM) of human periodontal ligament stem cells (hPDLSCs) on the proliferation and migration of tongue cancer Tca-8113 cells. Methods: hPDLSCs were isolated and cultured from healthy adult periodontal ligament tissues. The third generation hPDLSCs were replaced with serum-free medium at 80% confluence, and the supernatants were harvested after 24 h of further culture to obtain hPDLSCs-CM. The tongue cancer Tca-8113 cells were cultured in the conventional culture medium (control group) and the culture medium containing 50% hPDLSCs-CM (CM treatment group) respectively. Then the cell proliferation activity was detected by MTT assay, the cell cycle change was analyzed by FCM, Migration assay to detect changes in cell migration ability. Results: The isolated and cultured hPDLSCs expressed stem cell marker proteins Stro-1 and CD146 with obvious clonal formation and multi-directional differentiation. When hPDLSCs-CM was co-cultured with tongue cancer Tca-8113 cells on day 3, 5 and 7, the cell proliferation activity of CM-treated group was higher than that of untreated control group (P <0.01). On day 4, the proliferative index (PI) of CM group was significantly higher than that of control group (P <0.01). After cultured for 24 h, the number of transmembrane cells in the CM-treated group was significantly higher than that in the control group (P <0.01). Conclusion: hPDLSCs-CM can promote the proliferation of tongue cancer Tca-8113 cells and enhance its migration ability.