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目的从天山雪莲中筛选产黄酮的内生真菌,并对其进行鉴定。方法通过显色反应、TLC、HPLC法筛选产黄酮内生菌,标准曲线法测定黄酮含有量,ITS序列分析进行菌种鉴定。结果筛选到一株产黄酮的雪莲内生真菌YS101,鉴定为Aspergillus tabacinus,它在200 m L PDA培养基中所产的芦丁量大于9.0μg。结论本实验首次从天山雪莲中分离得到产芦丁的丝状真菌,可为黄酮的发酵法生产提供参考。
Objective To screen flavonoid endophytic fungi from Tianshan snow lotus and identify it. Methods Flavonoid endophytes were screened by color reaction, TLC and HPLC, the content of flavonoids was determined by standard curve method, and the strains were identified by ITS sequence analysis. Results A flavonoid-producing endophytic fungus YS101 was identified as Aspergillus tabacinus, which produced more than 9.0 μg of rutin in 200 mL PDA medium. Conclusions This experiment was the first to obtain rutin-producing filamentous fungi from Tianshanxilian and provide a reference for the fermentative production of flavonoids.