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为了探讨飞秒激光在兔眼角膜上行深板层切削的可行性,获得深板层切削的较适参数,并了解在水肿角膜上行深板层切削的准确性,将兔眼置于由同心圆扫描程序控制的三维平台上,将不同能量的飞秒激光(800 nm/50 fs)精确定位于角膜深板层,伴随平台移动激光便在角膜深板层切削。利用光学显微镜和扫描电镜(SEM)观察激光作用后角膜的形态学变化,并同徒手器械分离的板层角膜对比。当平台移动速度为200μm/s,激光能量为4.4μJ时切削表面比较平滑;当速度为300μm/s,能量为6.4μJ时切削表面较为平滑。激光组切削层面的光滑程度优于徒手器械分离组;当平台移动速度为200μm/s,能量为6.4μJ时飞秒激光同样可以完成在水肿角膜的400μm深度的聚焦切削,且切削表面较为光滑。
In order to investigate the feasibility of femtosecond laser ablation of deep lamellar upper layer in rabbit cornea and obtain the appropriate parameters of deep lamellar cutting and to understand the accuracy of upper lamellar ablation in eyelid cornea, Scanner-controlled three-dimensional platform, the different energy of femtosecond laser (800 nm / 50 fs) precise positioning of the corneal deep layer, with the platform to move the laser in the corneal deep layer of cutting. The morphological changes of the cornea after the laser action were observed by optical microscope and scanning electron microscope (SEM), and compared with the lamellar cornea separated by hand instruments. When the moving speed of the platform is 200μm / s and the laser energy is 4.4μJ, the cutting surface is relatively smooth. When the velocity is 300μm / s and the energy is 6.4μJ, the cutting surface is relatively smooth. The smoothness of the cutting surface of the laser group is better than that of the hand-free equipment. When the moving velocity of the platform is 200μm / s and the energy is 6.4μJ, the femtosecond laser can also focus and cut the 400μm depth of edema cornea, and the cutting surface is smoother.