论文部分内容阅读
目的 检测滋养细胞中TGF - β1mRNA表达水平 ,评估TGF - β1在妊高征中的作用。方法 用逆转录 -聚合酶链反应 (RT -PCR)检测正常妊娠胎盘滋养细胞 7例、重度妊高征胎盘滋养细胞 7例中TGF - β1mRNA表达水平 ,并通过紫外凝胶图像分析进行定量检测。结果 与正常滋养细胞比较 ,妊高征滋养细胞TGF - β1mRNA的表达强度显著增强 (t=- 4 .6 6 3,P <0 .0 5 )。结论 妊高征胎盘滋养细胞表达TGF - β1增多可能抑制其侵润能力。
Objective To detect the expression of TGF - β1 mRNA in trophoblast and evaluate the role of TGF - β1 in pregnancy induced hypertension. Methods The expression of TGF - β1 mRNA in 7 cases of normal gestational placental trophoblast cells and 7 cases of severe pregnancy induced placental trophoblast cells was detected by reverse transcriptase - polymerase chain reaction (RT - PCR) and quantitatively detected by UV gel image analysis. Results Compared with normal trophoblastic cells, the expression of TGF - β1 mRNA in PIH trophoblast cells was significantly increased (t = - 4.663, P <0.05). Conclusion The increased expression of TGF - β1 in placental trophoblasts of pregnancy induced hypertension may inhibit its invasive ability.