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目的观察研究神经干细胞(NSCs)移植入视神经部分损伤SD大鼠后闪光视觉诱发电位(F-VEP)的变化。方法24只健康成年SD大鼠随机分为NSCs移植组(N组)和对照组(C组),每组12只大鼠,两组均使用精确校准方法在大鼠右眼造成部分视神经损伤,左眼为正常对照。从胚胎SD大鼠海马分离NSCs,利用细胞培养和体内移植技术,将培养后的NSCs注入视神经损伤后N组大鼠玻璃体内,C组大鼠视神经损伤眼玻璃体内注入同等体积的PBS。以上两组分6个时间段,即损伤前、损伤时、损伤后1周、2周、3周、4周分别检测损伤视神经眼的F-VEP,记录P1波幅及峰潜时,并进行统计分析。结果N组及C组P1波幅随时间延长均不同程度降低,但前者趋势较后者缓和。自第2周开始N组波幅均高于C组,且差异有显著性；N组及C组P1峰潜时均随时间变化,在第3周时达到最长,第4周时P1峰潜时有缩短；自第1周开始N组峰潜时均较C组缩短,且差异均有显著性意义。结论NSCs移植入视神经部分损伤大鼠可部分改善视神经传导功能。
Objective To observe the changes of flash visual evoked potential (F-VEP) after transplantation of neural stem cells (NSCs) into part of optic nerve of SD rats. Methods Twenty-four healthy adult Sprague-Dawley rats were randomly divided into NSCs transplantation group (N group) and control group (C group), with 12 rats in each group. All of the rats were subjected to precise calibration to cause partial optic nerve injury in the right eye of rats, Left eye as a normal control. The NSCs were isolated from the hippocampus of embryonic SD rats. The cultured NSCs were injected into the vitreous of N rats after optic nerve injury, and the same volume of PBS was injected into the vitreous of C rats. The F-VEPs of the optic nerve eyes of the above two groups were detected before injury, at the time of injury, at 1 week, 2 weeks, 3 weeks and 4 weeks after the injury, respectively. The amplitude and peak latency of P1 were recorded and statistically analyzed analysis. Results The amplitudes of P1 in group N and group C decreased with time, but the tendency of the former tended to be more relaxed than the latter. The amplitudes of N group were higher than those of C group from the second week, and the differences were significant. The P1 peak latency of N group and C group changed with time and reached the longest at the third week, while the P1 peak at the fourth week Sometimes shortened; since the first week N peak latency was shorter than the C group, and the differences were significant. CONCLUSIONS Transplantation of NSCs into optic nerve partially damaged rats may partially improve optic nerve conduction function.