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应用直接电泳法和多克隆抗体捕捉法检测恶性疟和间日疟病人血样各４０份，正常对照血样２０份中恶性疟原虫特异性乳酸脱氢酶（ＬＤＨ－ｐ）。结果表明，两种方法检测恶性疟病人血样的阳性率分别为９０％和９５％；而间日疟和正常人血样中皆未检出ＬＤＨ－ｐ的活性。结果还表明，蛋白酶抑制剂可有效地保护ＬＤＨ－ｐ，而反复冻融则可使ＬＤＨ－ｐ受到破坏，检出率降低。应用直接电泳法和多克隆抗体捕捉法，在蛋白酶抑制剂存在的条件下，检测新鲜血样中ＬＤＨ－ｐ是诊断恶性疟的理想方法。
Plasmodium falciparum-specific lactate dehydrogenase (LDH-p) was detected in 40 blood samples of falciparum malaria and malaria patients and 20 control samples of blood samples by direct electrophoresis and polyclonal antibody capture. The results showed that the positive rates of the two methods for detecting blood samples of P. falciparum were 90% and 95%, respectively. However, no LDH-p activity was detected in blood samples of P. vivax and normal individuals. The results also show that protease inhibitors can effectively protect LDH-p, and repeated freezing and thawing can make LDH-p was destroyed, the detection rate decreased. Using direct electrophoresis and polyclonal antibody capture, the detection of LDH-p in fresh blood samples is an ideal method for the diagnosis of P. falciparum in the presence of protease inhibitors.