自1968年Berggard 首先从肾小管病变中分离和纯化β_2微球蛋白(β_2M),β_2M 的结构相继搞清.提纯β_2M 对纯化HLA 抗原提供了有用的工具.这里主要介绍从肾移植病人排异期尿中,通过凝胶过滤及抗人β_2M 亲和层析等分离纯化β_2M 的过程.一、材料和方法1.尿样浓缩:收集肾移植排异期尿,经5%磺基水杨酸测定尿蛋白定性为“++”以上排异期尿 Since 1968, Berggard first isolated and purified β_2-microglobulin (β_2M) from renal tubular lesions, and the structure of β_2M has been clarified one after another. Purification of β_2M provides a useful tool for the purification of HLA antigens. Urine, through gel filtration and anti-human β 2 M affinity chromatography separation and purification of β 2 M. Ⅰ Materials and Methods 1. Urine samples were concentrated: urine collected renal transplant rejection measured by 5% sulfosalicylic acid Urinary protein qualitative “+ +” above the exclusive urine