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目的：锌对热应激大鼠热休克蛋白７０ ｍ Ｒ Ｎ Ａ 表达的影响。方法：分别以高、中、低锌饲料（锌含量分别为９２２０、４５６１、２１７０ ｍ ｇ／ｋｇ 饲料）喂养３ 组大鼠，热暴露（干球４１５ ° Ｃ、湿球３２ ° Ｃ）３０ ｍ ｉｎ 时断头处死，应用 Ｎｏｒｔｈｅｒｎ 印迹杂交和斑点杂交检测热应激大鼠肝脏热休克蛋白７０ ｍ Ｒ Ｎ Ａ 的表达情况。结果：不经受热暴露的３ 组动物仅高锌组能检测到热休克蛋白７０ ｍ Ｒ Ｎ Ａ，而热暴露３０ ｍ ｉｎ 的３ 组动物均有热休克蛋白７０ ｍ Ｒ Ｎ Ａ 条带，且依锌水平不同，其表达强弱依次为高锌组强于中锌组，中锌组强于低锌组。结论：热应激时热休克蛋白７０ 基因的转录水平提高；锌通过活化热休克蛋白７０ 基因的转录而诱发热应激大鼠热休克蛋白７０ 的合成，从而使机体对热暴露产生更强的耐受力。
Objective: To investigate the effect of zinc on the expression of heat shock protein 70 m R N A in heat stress rats. Methods: Three groups of rats were fed with high, medium and low zinc diets (zinc diets of 9220, 4561 and 2170 mg / kg, respectively). Heat exposure (415 ° C, Wet bulb 32 ° C) was decapitated at 30 min, and the expression of heat-shock protein 70 m R N A was detected by Northern blotting and dot blotting. RESULTS: Heat shock protein 70 m R N A was detected only in the high zinc group in the three groups of animals that did not undergo thermal exposure, whereas the heat shock protein 70 m R N A band was observed in all three groups exposed to heat for 30 m in According to the different levels of zinc, the expression of zinc is higher than the zinc group, the zinc group is stronger than the low zinc group. CONCLUSIONS: The heat shock protein 70 gene transcriptional level increases. Zinc activates the transcription of heat shock protein 70 to induce the synthesis of heat-stressed rat heat shock protein 70, resulting in a stronger body heat exposure Tolerance.