论文部分内容阅读
采用甲基丙烯酸缩水甘油酯、丙稀酰胺为单体,N,N-亚甲基双丙稀酰胺为交联剂,十二醇、1,4-丁二醇和DMSO为制孔剂在毛细管中合成含有环氧基的整体柱,通过化学键合的方法将两性电解质(Ampholine)按照其等电点的顺序固定至整体柱中,制成新型固定化pH梯度整体柱(monolithicimmobilized pH gradient,M-IPG).以四种蛋白混合物对这种整体柱在毛细管等电聚焦中的应用加以评价,说明了M-IPG对蛋白质的分离特征.与先前研究的聚丙烯酸酯基质固定化pH梯度整体柱相不同,这种新型整体柱具有更好的亲水性,能够有效防止蛋白吸附,非常适用于蛋白质等生物大分子的分离分析.
Adopting glycidyl methacrylate, acrylamide as monomer, N, N-methylenebisacrylamide as crosslinking agent, dodecanol, 1,4-butanediol and DMSO as pore-forming agent in capillary The monolithic immmobilized pH gradient monolithic column (M-IPG) was synthesized by immobilizing Ampholine into the monolithic column in the order of its isoelectric point by chemical bonding. ). The evaluation of this monolithic column for capillary isoelectric focusing using a mixture of four proteins demonstrated the M-IPG separation profile of the protein, which is different from the polyacrylate matrix-immobilized pH gradient monolithic columns previously studied , This new monolithic column has better hydrophilicity, can effectively prevent protein adsorption, and is very suitable for the separation and analysis of biological macromolecules such as proteins.