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目的研究苦参碱和氧化苦参碱对抗原攻击所致血管收缩的影响。方法腹腔注射牛血清白蛋白建立预致敏的豚鼠模型,21d后制备离体豚鼠主动脉环,用0.04mg.mL-1牛血清白蛋白诱发血管过敏反应,记录血管张力的改变。观察苯海拉明、苦参碱和氧化苦参碱对抗原攻击所致血管收缩的影响,并观察capsazepine等对不同剂量苦参碱和氧化苦参碱作用的影响,分析其对血管过敏性损伤保护作用的机制。结果抗原攻击能诱导豚鼠预致敏的离体胸主动脉环收缩,苯海拉明(H1受体阻断剂)能显著抑制抗原攻击所致的血管收缩;氧化苦参碱(3.3×10-4～3.3×10-3mol·L-1)能显著降低抗原攻击所致血管的收缩效应,瞬时受体电位香草酸亚型(Transient Receptor Potentiol Vanilloid 1,TRPV1)受体阻断剂capsazepine(10μmol·L-1)能消除氧化苦参碱对血管收缩的抑制效应;苦参碱(1×10-3、3.3×10-3mol·L-1)能显著降低抗原攻击所致血管的收缩效应,此作用不受capsazepine(10μmol·L-1)的影响。结论氧化苦参碱能显著抑制血管过敏反应时血管的收缩,其作用与激活TRPV1受体有关;较高浓度苦参碱也能显著抑制血管过敏反应时血管的收缩,其作用与TRPV1受体无关。
Objective To investigate the effects of matrine and oxymatrine on vasoconstriction induced by antigen challenge. Methods The pre-sensitized guinea pig model was established by intraperitoneal injection of bovine serum albumin. After 21 days, the isolated aortic rings of guinea pigs were induced. 0.04mg.mL-1 bovine serum albumin was used to induce vascular anaphylaxis and the changes of vascular tone were recorded. To observe the effect of diphenhydramine, matrine and oxymatrine on vasoconstriction induced by antigen challenge, and to observe the effects of capsazepine on different doses of matrine and oxymatrine, and to observe their effects on vascular atypical injury Mechanism of protection. Results Antigen challenge induced contractile contraction of prothoracic isolated thoracic aorta in guinea pigs. Diphenhydramine (H1 receptor blocker) significantly inhibited vasoconstriction induced by antigen challenge. Oxymatrine (3.3 × 10- 4 ~ 3.3 × 10-3mol·L-1) could significantly reduce the contractile effect of blood vessels induced by antigen challenge. Transient receptor Potentiol Vanilloid 1 (TRPV1) receptor blockers, capsazepine (10μmol · L-1) could abolish the inhibitory effect of oxymatrine on vasoconstriction. Matrine (1 × 10-3,3.3 × 10-3 mol·L-1) could significantly reduce the contractile effect of blood vessels induced by antigen attack The effect is not affected by capsazepine (10 μmol·L-1). Conclusion Oxymatrine can significantly inhibit vascular contraction during vascular anaphylactic reaction, which is related to the activation of TRPV1 receptor. Higher concentrations of matrine can significantly inhibit vascular contraction during vascular anaphylaxis and its effect is not related to TRPV1 receptor .