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为给从分子水平上揭示枣核发育的机理及培育无核枣优良品种提供理论基础,以“金丝小枣”幼果为试材,对枣果实中苯丙氨酸解氨酶(PAL)基因进行了克隆,并进行了生物信息学分析。用分子克隆方法首次从枣树中克隆了苯丙氨酸解氨酶基因部分cDNA序列,并命名为ZjPAL。ZjPAL长1 954 bp,编码559个氨基酸。通过核苷酸和氨基酸序列多重比对,发现ZjPAL与其它植物的PAL基因高度同源。ZjPAL编码的氨基酸序列包含与水稻、玉米PAL相同的脱氨基位点和催化活性位点。
In order to provide a theoretical basis for revealing the mechanism of jujube development at the molecular level and cultivating fine varieties without nuclear jujube, the effects of phenylalanine ammonia lyase PAL) gene was cloned and analyzed by bioinformatics. The partial cDNA sequence of phenylalanine ammonia-lyase gene was cloned from jujube tree for the first time by molecular cloning method and named as ZjPAL. ZjPAL is 1 954 bp long and encodes 559 amino acids. By multiple alignment of nucleotide and amino acid sequences, ZjPAL was found to be highly homologous to PAL genes of other plants. The amino acid sequence encoded by ZjPAL contains the same deamination sites and catalytically active sites as PAL and maize PAL.