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本文对室内长期饲养的小菜蛾(Plutella xylostella L.)敏感品系和田间采集的抗性种群体内的艾氏剂平氧化酶及细胞色素P-450进行了比较研究。结果证明,艾氏剂环氧化酶在感性和抗性小菜蛾间存在着量及质的差异。抗性种群的艾氏剂环氧化酶的Vmax和Km值分别为感性品系的5.4%和6.5倍。抗性种群的细胞色素P-450的含量是感性品系的1.1—1.3倍。艾氏剂环氧化酶在量上及质上的差异及细胞色素P-450含量的提高是导致小菜蛾抗药性发生与发展的重要机制之一。而且质的差异较之量的差异可能起着更为重要的作用.
In this paper, we compared the aldrin-like oxidase and cytochrome P-450 in long-term indwelling Plutella xylostella L. susceptible and field-collected resistant populations. The results showed that aldrin epoxidase in quantitative and resistant diamondback moths there is a qualitative and quantitative differences between. The Vmax and Km values of aldrin epoxidase in resistant populations were 5.4% and 6.5 times that of susceptible strains, respectively. The content of cytochrome P-450 in the resistant population was 1.1-1.3 times that of the susceptible strain. The quantitative and qualitative difference of aldrin epoxidase and the increase of cytochrome P-450 content are one of the important mechanisms that lead to the occurrence and development of drug resistance in Plutella xylostella. Moreover, the difference of quality may play a more important role than the difference of quantity.