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目的观察慢性再生障碍性贫血(CAA)患者的T淋巴细胞亚群(CD4,CD8,CD4/CD8)、γ-干扰素(IFN-γ)、肿瘤坏死因子-α(TNF-α)、白细胞介素-2(IL-2)以及红细胞C3b受体花环率(RBC-C3bRR)和红细胞免疫复合物(RBC-C IC)的变化,探讨机体免疫功能和CAA发病的关系。方法T淋巴细胞亚群(CD4,CD8)采用流式细胞仪进行检测,血清IFN-γ、TNF-α、IL-2的测定采用酶联免疫吸附法(ELISA),RBC-C3bRR和RBC-C IC测定采用红细胞酵母花环法。结果与对照组相比,CD4、CD4/CD8以及RBC-C3bRR显著降低(P<0.05和P<0.01)。而CD8和IFN-γ、TNF-α、IL-2水平则显著升高(P<0.05和P<0.01)。结论CAA患者表现多种免疫功能改变。监测CAA患者的白细胞及红细胞免疫活性有助于了解病情变化。
Objective To observe the changes of T lymphocyte subsets (CD4, CD8 and CD4 / CD8), IFN-γ, TNF-α and leukocyte in patients with chronic aplastic anemia (CAA) (RBC-C3bRR) and erythrocyte immune complex (RBC-C IC) in patients with chronic hepatitis B and explore the relationship between immune function and CAA. Methods T-lymphocyte subsets (CD4, CD8) were detected by flow cytometry. Serum levels of IFN-γ, TNF-α and IL-2 were measured by enzyme linked immunosorbent assay (ELISA), RBC-C3bRR and RBC-C IC assay using red yeast cell wreath method. Results Compared with the control group, CD4, CD4 / CD8 and RBC-C3bRR were significantly decreased (P <0.05 and P <0.01). While the levels of CD8, IFN-γ, TNF-α and IL-2 increased significantly (P <0.05 and P <0.01). Conclusion CAA patients showed a variety of immune function changes. Monitoring leukocyte and erythrocyte immune activity in patients with CAA helps to understand the condition changes.