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目的探索依达拉奉对脑缺血再灌注大鼠的血管内皮生长因子(VEGF)和血管生成素-1(Ang-1)的影响。方法采用大脑中动脉闭塞法建立大鼠脑缺血再灌注模型,将雄性SD大鼠随机分为3组:假手术组、模型组和依达拉奉组,每组19只。HE染色观察梗死灶周围的病理改变,透射电镜下观察梗死灶周围脑组织的毛细血管内皮形态变化,激光多普勒微循环血流仪监测微循环血流量,采用免疫组化检测脑组织VEGF和Ang-1的表达。结果依达拉奉组的组织病理损伤和血管内皮细胞损伤要轻于模型组,微循环血流量(47.32±6.58)明显高于模型组(40.51±7.96)(P<0.05)。模型组大脑组织的VEGF和Ang-1[(0.124±0.021),(0.099±0.014)]均明显高于假手术组[(0.118±0.018),(0.095±0.016)](均P<0.05),而依达拉奉组大脑组织的VEGF和Ang-1[(0.147±0.019),(0.124±0.021)]均明显高于模型组[(0.136±0.023),(0.118±0.018)](均P<0.05)。结论依达拉奉能上调缺血再灌注大鼠脑组织的VEGF和Ang-1来保护梗死灶周围毛细血管的内皮细胞。
Objective To explore the effect of edaravone on the expression of vascular endothelial growth factor (VEGF) and angiopoietin-1 (Ang-1) after cerebral ischemia-reperfusion in rats. Methods The cerebral ischemia-reperfusion model was established by middle cerebral artery occlusion. The male SD rats were randomly divided into three groups: sham operation group, model group and edaravone group, with 19 rats in each group. The pathological changes around the infarct were observed by HE staining, the changes of capillary endothelial morphology in the brain tissue around the infarct were observed under a transmission electron microscope, the microcirculation blood flow was monitored by laser Doppler flowmeter, the VEGF and Ang-1 expression. Results The edaravone group had less histopathological damage and vascular endothelial cell injury than the model group. The microcirculation blood flow (47.32 ± 6.58) was significantly higher than that of the model group (40.51 ± 7.96) (P <0.05). The levels of VEGF and Ang-1 [(0.124 ± 0.021) and (0.099 ± 0.014)] in model group were significantly higher than those in sham group [(0.118 ± 0.018) and (0.095 ± 0.016), respectively] However, the levels of VEGF and Ang-1 [(0.147 ± 0.019) and (0.124 ± 0.021)] in edaravone group were significantly higher than those in model group [(0.136 ± 0.023) and (0.118 ± 0.018) 0.05). Conclusion Edaravone can up-regulate the expression of VEGF and Ang-1 in the brain tissue of ischemic-reperfusion rats to protect the capillary endothelial cells around the infarct.