【摘 要】
：
随着水产养殖病害问题日益严重，抗生素和药物使用对环境造成的危害越来越大，开展绿色生态防控技术研究是实现水产健康养殖的重要途径。该研究从健康草鱼 (Ctenopharyngodon idella) 肠道分离获得8株乳酸菌 (Lactobacillus)，经生化反应和16S rRNA鉴定，均为植物乳杆菌 (L. plantarum)。生物学特性评价结果表明植物乳杆菌分离株Y190430的发酵性能最好，且具有良好的抗生素敏感性；与植物乳杆菌标准株ATCC8014和商品乳酸菌相比较，分离株Y190430对酸、碱、
【机 构】
：
上海海洋大学/水产科学国家级实验教学示范中心,上海,201306;中国水产科学研究院珠江水产研究所/农业农村部渔药创制重点实验室/广东省水产动物免疫技术重点实验室,广东,广州,510380
论文部分内容阅读
随着水产养殖病害问题日益严重，抗生素和药物使用对环境造成的危害越来越大，开展绿色生态防控技术研究是实现水产健康养殖的重要途径。该研究从健康草鱼 (Ctenopharyngodon idella) 肠道分离获得8株乳酸菌 (Lactobacillus)，经生化反应和16S rRNA鉴定，均为植物乳杆菌 (L. plantarum)。生物学特性评价结果表明植物乳杆菌分离株Y190430的发酵性能最好，且具有良好的抗生素敏感性；与植物乳杆菌标准株ATCC8014和商品乳酸菌相比较，分离株Y190430对酸、碱、盐、温度等环境胁迫具有更好的耐受性，同时具有更快的产酸速率，并可通过代谢产物抑制病原菌生长，体外抑菌试验表明分离株Y190430对常见水产病原菌的拮抗能力更强。“,”As the problem of aquaculture diseases is growing, the harm of using antibiotics and drugs is more and more serious so the research on ecological control technology is an important way to achieve healthy aquaculture. In this study, eight lactobacillus strains were isolated from the intestinal tract of healthy grass carps (Ctenopharyngodon idella). The identification result indicates that all the eight strains were Lactobacillus plantarum. The results of biological characteristics evaluation show that L. plantarum strain Y190430 had a high growth rate, and it was sensitive to common antibiotics. In addition, compared with the standard strain and commercial lactobacillus, L. plantarum Y190430 represented better tolerance to environmental stress such as acid, alkali, salt and temperature. Besides, it had a higher acid production rate and inhibited the growth of pathogenic bacterias better. The results provide references for the research on ecological control of aquatic diseases.
其他文献
Despite emerging contemporary biotechnological methods such as gene- and stemcell-based therapy, there are no clinically established therapeutic strategies for neural regeneration after spinal cord injury. Our previous studies have demonstrated that trans
The axon initial segment (AIS) region is crucial for action potential initiation due to thepresence of high-density AIS protein voltage-gated sodium channels (Nav). Nav channels comprise several serine residues responsible for the recruitment of Nav chann
Human induced pluripotent stem cells (hiPSCs) are multipotent stem cells genetically reprogrammed using transcription factors, such as Sox2, c-Myc, Oct3/4 and Klf4 (Takahashi and Yamanaka, 2006) from fibroblasts, derived from either patient or control ind
Functional electrical stimulation is an effective way to rebuild hindlimb motor function after spinal cord injury. However, no site map exists to serve as a reference for implanting stimulator electrodes. In this study, rat models of thoracic spinal nerve
黄鳍棘鲷 (Acanthopagrus latus) 是中国具有重要商业价值的海水鱼类之一，对其精子开展冷冻保存可为黄鳍棘鲷育种提供一定的技术支持，可有效预防其种质资源衰退，保持其养殖业的可持续发展。该研究以黄鳍棘鲷精子为实验材料，对其精子冷冻保存的稀释液、葡萄糖浓度、抗冻剂种类及浓度、精子与保护液稀释比例和降温程序等进行了筛选优化。结果表明，以含10 g·L−1葡萄糖、5%乙二醇的MPRS溶液与精子按1∶2的比例稀释，4 ℃平衡30 min，液氮面上5 cm熏蒸5 min，最后投入液氮保存2 h，将黄鳍
Trauma to the peripheral nervous system often results in loss of motor and sensory functions of the affected area of the body, leading to a series of functional impairments (Allodi et al., 2012). Injuries to peripheral nerves initiate a series of complex
文章根据1986—1987年和 2018—2019年珠江河口棘头梅童鱼 (Collichthy lucidus) 的调查数据，对其群体结构、生长、摄食等主要生物学特征的年代际变化进行了研究。结果表明，这2个调查时间段内棘头梅童鱼群体优势体长组均为90~110 mm，平均体长分别为 (95±13) mm和 (93±18) mm。2个年代际的平均体长无显著差异 (P>0.05)，但优势体长组个体数占比降低 (P0.05)。2018—2019年棘头梅童鱼雌、雄个体的平均肥满度和平均摄食等级均存在显著差异 (P0
鳞鳍叫姑鱼 (Johniusdistinctus) 和皮氏叫姑鱼 (J. belangerii) 是我国东海的小型经济鱼类，且为生态系统中重要的饵料鱼类，研究分析其生物学特征对促进东海生态系统和渔业的科学管理具有重要意义。根据2015年11月至2018年11月浙江南部近海渔业资源季度调查数据，运用线性混合效应模型 (Linear mixed effects model, LMEM) 研究了这2种叫姑鱼的生长特征及其异质性；通过ELEFAN法估算了2种叫姑鱼的极限体长L∞、趋近极限体长的相对生长速率k、理论
为了解长江下游鲢 (Hypophthalmichthys molitrix) 遗传多样性和遗传结构分布，该研究利用微卫星分子标记结合毛细管电泳技术，通过对2017—2019年长江湖口、安庆、芜湖、当涂、镇江、靖江、张家港和常熟8个江段鲢野生群体共327 份DNA进行基因分型与遗传多样性相关分析，比对了其遗传多样性、遗传分化和遗传结构情况。结果显示，长江下游8 个群体总体遗传多样性水平较高，但群体内缺乏足够的杂合子，8个群体等位基因数 (Na)介于6.0~12.3，有效等位基因数(Ne)介于3.94~6.1